叶云峰, 杜婵娟, 覃斯华, 柳唐镜, 洪日新, 付岗, 李桂芬, 黄金艳. 2018: 甜瓜尾孢叶斑病病原分离与鉴定. 南方农业学报, 49(3): 476-480. DOI: 10.3969/j.issn.2095-1191.2018.03.10
引用本文: 叶云峰, 杜婵娟, 覃斯华, 柳唐镜, 洪日新, 付岗, 李桂芬, 黄金艳. 2018: 甜瓜尾孢叶斑病病原分离与鉴定. 南方农业学报, 49(3): 476-480. DOI: 10.3969/j.issn.2095-1191.2018.03.10
shu
YE Yun-feng, DU Chan-juan, QIN Si-hua, LIU Tang-jing, HONG Ri-xin, FU Gang, LI Gui-fen, HUANG Jin-yan. 2018: Isolation and identification of pathogen causing Cercospora leaf spot of muskmelon. Journal of Southern Agriculture, 49(3): 476-480. DOI: 10.3969/j.issn.2095-1191.2018.03.10
Citation: YE Yun-feng, DU Chan-juan, QIN Si-hua, LIU Tang-jing, HONG Ri-xin, FU Gang, LI Gui-fen, HUANG Jin-yan. 2018: Isolation and identification of pathogen causing Cercospora leaf spot of muskmelon. Journal of Southern Agriculture, 49(3): 476-480. DOI: 10.3969/j.issn.2095-1191.2018.03.10
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甜瓜尾孢叶斑病病原分离与鉴定

Isolation and identification of pathogen causing Cercospora leaf spot of muskmelon

    摘要
  • 摘要: 目的明确近年来引起广西大棚厚皮甜瓜叶斑病的病原,为该病害的有效防治提供参考依据.方法采用单孢分离法直接从罹病厚皮甜瓜叶片上分离病原菌,以离体叶片接种法验证病原菌的致病性.对致病菌的菌落、产孢结构、分生孢子等形态特征进行观察,同时对致病菌的rDNA-ITS基因序列进行同源性比对分析.结果病原菌形态特征与瓜类尾孢(Cercospora citrullina)基本一致,rDNA-ITS基因序列与瓜类尾孢的同源性达99%.结合病原菌的形态学和分子生物学特征,将该病害的病原菌鉴定为瓜类尾孢.结论近年在广西大棚厚皮甜瓜产区严重发生的甜瓜叶斑病由瓜类尾孢引起,该病是广西厚皮甜瓜上的一种新病害.

     

    Abstract: ObjectiveThe pathogen causing leaf spot disease on muskmelon in greenhouse in Guangxi in recent years was clarified to provide reference for the effective control of the disease.MethodThe pathogenic fungi were isolated by single spore isolation method from leaves of diseased muskmelon.The pathogenicity of pathogenic fungi was verified by inoculation of detached leaves.Furthermore,the colony,conidiophores and conidia characteristics of the pathogen were observed.Homology analysis on rDNA-ITS gene sequences of the pathogenic fungi was conducted.ResultThe morpho-logical characteristics of the pathogen were consistent with Cercospora citrullina. And its rDNA-ITS gene sequences showed 99% similarity with C. citrullina. Based on the morphological and molecular characteristics,the pathogen was identified as C. citrullina.ConclusionThe serious leaf spot appeared on muskmelon in greenhouse in Guangxi these years is caused by C.citrullina.It is a new disease on muskmelon in Guangxi.

     

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