Abstract: ObjectiveAn easy and efficient process to obtain mangiferin from mango leaves was set up to provide raw materials and technical support for developing mangiferin products.MethodThrough five steps(material crushing→ethylacetate(EAC)impurity-removing→methanol(Met)extraction→equivalent extract washing→crystal precipitation),mangiferin was isolated from mango leaves.By means of single factor test and L9(34)orthogonal test,the technical param-eters including extraction time,ultrasonic power,extraction frequency and extraction temperatue were optimized.ResultThe optimized process for extracting mangiferin from mango leaves by EAC impurity-removing method was as follows:mango leaves were crushed and sifted,added 3 mL/g EAC,soaked for 4 h in sealed container,extracted by ultrasonic vi-brating for 20 min(50℃,350 W),filtrated through 100 mesh for 3 times at room temperature;added 100% Met in the residue on the basis of 3 mL/g,extracted by ultrasonic vibrating(20 min,50℃,350 W),filtrated through 100 mesh while its was hot;mixed the extractions,condensed by vacuum decompression to get extractum with 25.5% mangiferin;added 100% Met in the extractum on the basis of 4 mL/g,stirred washing for 5 min at room temperature,let it still for 10 min,fil-trated through 100 mesh for 3 times,dried the residue at 60℃to get the crude product;added 100% Met in the crude prod-uct on the basis of 4 mL/g,stirred washing for 5 min at 50℃,put it still for 8 h at 6℃,dried the residue at 60℃,crystal-lized for twice to obtain pure product with purity>98.00% and recovery rate 79.10%.Using this process,crude mangifer-in product and pure mangiferin product could obtain.The purity of crude mangiferin product>64.00% with recovery rate of 83.90%,and purity of pure mangiferin product >98.00% with recovery rate of 66.40.ConclusionThe optimized EAC impurity-removing process is an easy,low-cost and efficient operation to extract mangiferin from mango leaves,and can be applied in real mangiferin production.