Abstract: ObjectiveThe most suitable ecological environment for Gracilaria salicornia growth was researched in order to provide reference for its artificial cultivation. MeasureEffects of temperature, light intensity and salinity on varia-tion of growth rate, chlorophyll a(Chla), carotenoid(Car), phycoerythrin(R-PE), phycocyanin(R-PC), soluble protein, superoxide dismutase(SOD) and malondialdehyde(MDA) were analyzed by indoor single factor experiment. ResultThe suitable growth temperature for G. salicornia was 24-32 ℃, and the optimum temperature was 28 ℃; the suitable light in-tensity was 3000-9000 lx, and the most suitable light intensity for growth was 6000 lx; the suitable salinity was 20‰-35‰and the optimum salinity for growth was 30‰. Under the suitable conditions, growth rate of G. salicornia was high. And it contained high phycobiliprotein, soluble protein and SOD contents, low MDA content. It was inferred that G. salicornia suffered less from environment stree than oxidative stress. ConclusionG. salicornia can adapt to the environment condi-tions of high temperature, low light intensity and medium-and-high salinity. Therefore, it can be cultured naturally in sum-mer in the southern sea area of China.