Abstract: ObjectiveIn the present study, bacterial community diversity in rhizosphere soil of peppers with phytoph-thora blight was studied to provide reference for control of pepper phytophthora blight. MethodRhizosphere soil of peppers dying from phytophthora blight was collected from Changsha, Jishou and Yongzhou in Hunan. 16S rDNA-V4 regions of sample genome DNA were sequenced by IlluminaMiSeq high-throughput sequencing to analyze bacterial community diver-sity in rhizosphere soil of peppers with phytophthora blight in different areas. ResultBacteria OTU number and propor-tions of unique OTU of rhizosphere soil of peppers with phytophthora blight of three areas were different. The proportion of Yongzhou accounted for 30.18% of the total, and that of Jishou accounted for 41.83% of the total and that of Changsha accounted for 24.07% of the total. Shared OTU of the three areas accounted for 14.95% of all detected OTU. Alpha diversity index were the lowest in Changsha and the highest in Jishou. At phylum level, the microbial composition was similar in rhi-zosphere soil of peppers with phytophthora blight of three areas, the dominant bacteria were Acidobacteria, Proteobacteria, Actinobacteria and Chloroflexi, but relative abundance showed some differences. ConclusionThere is difference in bac-terial community diversity in rhizosphere soil of peppers dying from phytophthora blight in Yongzhou, Jishou and Chang-sha, the diversity of Jishou is the highest while that of Changsha is the lowest. The microbial composition is similar in rhizo-sphere soil of peppers with phytophthora blight of the three areas, but the relative abundance is different.