Abstract: Objective Effects of different temperatures and gibberellin (GA3 ) with different concentrations on seed germination and seedling sprout tillers growth of Lotus corniculatus L. were studied in order to provide theoretical basis for high yield cultivation and comprehensive application of L. corniculatus L. MethodSeed germination test of L. corniculatus L. was carried out at 10, 15, 20 and 25 ℃ respectively,and seed soaking germination test with 100.0, 200.0, 300.0, 400.0 and 500.0 mg/L GA3 solution was carried out under constant temperature condition of 20 ℃.The germination rate, germination potential, germination index,seedling sprout tillers and root growth of seedlings in the two tests were comparatively analyzed. ResultThe germination rate(64.44%) of L. Corniculatus L. seeds was the highest at 10 ℃. Germination potential(40.00%) and germination index(7.17) were the highest at 20 ℃. Germination rate(42.22%) was the lowest when treated by 25 ℃,and it was significantly different(P<0.05,the same below) from that treated by 10 ℃. The germination potential(10.00%) and germination index(0.50) was the lowest at 10 ℃,and was significant different from those treated at 15,20 and 25 ℃. Under the condition of constant temperature at 20 ℃, for seedlings in 100.0 mg/L GA3 treatment, germination rate(52.22%),germination potential(45.56%) and germination index(7.41) of L. corniculatus L.seeds were significantly higher than those in 300.0,400.0 and 500.0 mg/L GA3 treatments. The average net sprout tillers were the highest(4.73) in autumn. The average net sprout tillers were 2.23 in spring, 3.40 in summer and 2.53 in winter.ConclusionThe best germination effects of L. corniculatus L. seeds is achieved in 100.0 mg/L GA3 soaking treatment for 24 h at 20 ℃ constant temperature. In this treatment, the number of sprout tillers growth in autumn is the most. Therefore, it can be applied in L. corniculatu L. cultivation.