Abstract: ObjectiveMedium for symbioses establishment between mycorrhizal fungi and Prunus pseudocerasus seedlings was screened in order to provide reference for improving transplanting survival rate of tissue-cultured P. pseudo-cerasus seedlings. MethodTissue-cultured P. pseudocerasus seedlings in media DE, 1/2DE, 1/4DE and 1/8DE were inoculated. Root infection rate,plant height,leaf enzyme activity,branch number of root and survival rate of tissue-cultured seedlings were determined after 30, 40, 50 and 60 d of inoculation. Culture medium suitable for symbiosis between mycorrhizal fungi and P. pseudocerasus seedlings was selected. ResultThe seedlings in DE and 1/2DE media died 30 and 50 d after mycorrhizal fungi inoculation, seedlings in media 1/4DE and 1/8DE remained 20.0%and 70.0%survival rates respectively. Root infection rate,branch number of root,plant height and leaf peroxidase activity of P. pseudocerasus seedlings gradually increased in 1/4DE and 1/8DE media 30 d after treatment and reached the peaks 60 d after inoculation. ConclusionInoc-ulation of fungi during seedling culture process can make the root mycorrhizal, which is the complicated reaction among tissue-cultured seedlings, mycorrhizal fungi and medium. 1/8DE culture medium is beneficial for symbioses establishment between P. pseudocerasus seedlings and mycorrhizal fungi.