Abstract: ObjectiveThe present experiment was conducted to study the effects of different slow-release fertilizers on biological effect and soil fertility for spring potato in Guizhou, which provided reference for the simplified cultivation, "re-duce the application of fertilizer and pesticide" and "save water, fertilizer and pesticide" comprehensive technology inte-gration of spring potato in Guizhou. MethodIn the field test, seven different slow release fertilizers and common compound fertilizers were used. The effects of different slow release fertilizers on yield, tuber traits and soil fertility in the same amount of fertilizer(N 240 kg/ha, P2O5120 kg/ha and K2O 270 kg/ha) were studied. ResultCompared with control, the slow re-lease fertilizers Jinzhengda, Nuotaike, Yangfeng and Shikefeng could significantly increase the yield of spring potato by 19.1%-24.6%(P<0.05, the same below). Compared with control, in slow release fertilizer treatments, tuber yield per plant increased by 2.6%-73.1% and the large and medium-sized tuber percentage increased by 6.6%-15.1%. The effects of Jinzhengda were the best. While slow release fertilizers Xiyang, Wofute and Hualong decreased the yield significantly. Compared with the control, application of slow release fertilizer could increase the soil pH(3.8%-7.5%), organic matter (27.1%-59.1%), total nitrogen(14.3%-33.3%), alkali-hydrolyzable nitrogen(8.7%-47.8%), total potassium(7.0%-26.3%) and available potassium(8.8%-42.1%). But total phosphorus and available phosphorus content decreased by 7.1%-21.4%and 35.6%-58.0%respectively. ConclusionSlow release fertilizers can increase the yield of spring potato and improve soil fertility obviously. But the effects of different slow release fertilizers are various. Overall, slow release fertilizers Jinzhengda, Nuotaike, Yangfeng and Shikefeng have the optimal yield increasement, and can be promoted in local area.