Abstract: ObjectiveOviposition behavior and best mating time of stocks of Liangguang No.2 were studied in order to provide reference for shortening egg age gap and improving egg producing management technique. MethodFemale moths of four varieties of stocks of Liangguang No.2 for double cross(Furong×932,932×Furong,Xianghui×7532,7532×Xianghui) were taken as test materials. Egg amount percentage of the total amount at different times after mating at different times, egg number per moth and poor egg rate of different varieties were researched, in order to find out the oviposition behavior of stocks for double cross by different mating patterns. ResultThere were differences in oviposition behavior between differ-ent mating times for the same variety. Chinese varieties(Furong×932 and 932×Furong) oviposition graph presented obvious wavy, it showed obvious oviposition ups and downs. When mating at 8:00 or 9:00, the oviposition was not so concentrated with a long durable time;while mating at 10:00 or 11:00, moth spawned rapidly and reached oviposition peak after departing, the oviposition process was relatively concentrated. Japanese varieties (Xianghui×7532,7532×Xianghui) oviposition regulation mating at different times were similar, and oviposition amount remained steady and spawned rapidly. During each oviposition period, oviposition amount declined as time extended. For the four varieties, different mating times had insignificant difference on egg number per moth and poor egg rate. But there existed significant difference on egg number per moth and poor egg rate between different varieties based on two-factor variance analysis(P<0.01). ConclusionDifferent mating times has influence on opvisition peak time and concentration, but no influence on egg number per moth and poor egg rate. The occurrence rules were different. In actual cultivation, time point of mating, departing, releasing moth and taking back moth should be adjusted based on opvisition behavior. Silkworm egg protection and pickling can also refer to opvisition behavior, so as to reduce egg age gap, enhance degree of hatching uniformity and hatching rate.