响应面法优化蔗梢多糖超声波提取工艺

Optimization on ultrasonic extraction of polysacchrides from sugarcane (Saccharum officinarum L.) tops by response surface analysis

摘要: 【目的】优化蔗梢多糖的提取工艺条件,为蔗梢多糖的开发利用提供技术参考。【方法】以多糖提取率和DPPH自由基清除率为指标,采用响应面法分析优化超声波提取蔗梢多糖的工艺条件。【结果】超声波提取蔗梢多糖最佳工艺为:超声功率640 W,提取温度69℃,提取时间29 min,在此条件下,其多糖提取率为4.68%,DPPH自由基清除率为74.89%。【结论】采用响应面法优化蔗梢多糖超声波提取工艺具有较高的可行性。

Abstract: ObjectiveThe extraction parameters of polysaccharides in sugarcane (Saccharum officinarum L.) tops were optimized to provide references for utilizing sugarcane top polysaccharides. MethodWith polysaccharides yield and DPPH scavenging activity as the indicators, response surface analysis (RSA) was used to optimize the ultrasonic extraction conditions for polysaccharides from sugarcane tops. ResultThe optimal conditions were ultrasonic power of 640 W, ex-traction temperature of 69 ℃, and extraction time of 29 min, under which the extraction yield and the DPPH scavenging rate of polysaccharides was 4.68% and 74.89%, respectively. ConclusionThe response surface methodology was a rea-sonable method to optimize the extraction parameters of sugarcane top polysaccharides.