Abstract: ObjectiveThe second fruiting technology of Yeniang No.2 Vitis quinquangularis Rehd. with hermaphrodite flower was studied to provide theoretical references for pruning the second fruits of Vitis quinquangularis Rehd. scientifically and properly. MethodSecond fruiting production tests of summer buds and winter buds in Yeniang No.2 were conducted to investigate the relationship among bearing branch node, branch roughness and winter bud flowering. The first and second fruit yield and quality traits were comparatively analyzed. ResultWinter bud flowering and fruit character were better than those of summer bud. Pruning time was chosen in late June to middle July. Bearing branch roughness was greater than 1.0 cm, and winter buds sprouted from 7-12 node showed strong sprouting, flowering and fruiting ability. The second fruits had superior brewing wine quality with high sugar and acid and strong flavor. ConclusionBearing branch roughness should be bigger than 1.0 cm and the 7-12 node should be maintained for pruning in late June to middle July. Under above-mentioned conditions, fruiting of winter bud could be promoted and high-yield and top quality second fruits could be produced.