Abstract: ObjectiveThe tissue culture and rapid propagation of a new potato variety Guinongshu 1 were studied to pro-vide technical support for industrializing production of virus-free potato seeds.MethodThe stem tip detoxification method was adopted and stem tip was used as explants. The single factor (3% NaClO and 0.1% HgCl2) and two factors (75% alcohol and 0.1%HgCl2) disinfection treatments were conducted at different sterilization times. Stem tip with the size of 0.3-0.7 mm were induced into seedlings in induction medium with different concentrations of GA3, 6-BA and combinations of 6-BA and NAA. The induced single lateral bud was cut and inoculated in MS culture medium of single factor and two factors combina-tion added with 6-BA and PP33 to proliferate. ResultThe sterilization effect of two factors of 75%alcohol+0.1%HgCl2 was better than single factor of 3%NaClO or 0.1%HgCl2, and the pollution rate declined to 21.67%. Both GA3 and 6-BA could in-duce germination of stem tip with an induced trend of increase-decrease. The induction rate was as high as 53.67%for combi-nation of 1.5 mg/L 6-BA and 0.2 mg/L NAA. At multiplication stage, the multiplication rate of combination treatment adding 0.1 mg/L PP333 and 2.5 mg/L 6-BA was as high as 6.59 times and the seedlings grew better with strong stems and leaves, and normal color. ConclusionFor Guinongshu 1 explants, the best sterilization treatment was 75%alcohol 2 min+0.1%HgCl2 11 min and the best stem tip induction formula was MS+1.5 mg/L 6-BA+0.2 mg/L NAA. The best multiplication medium was MS+2.5 mg/L 6-BA+0.1 mg/L PP333.