何奇松, 孙翔翔, 易春华, 蒋家霞, 付薇, 马琳, 熊毅. 2010: 白鹭源禽Ⅰ型副粘病毒L基因的克隆与序列分析. 南方农业学报, 41(5): 481-484. DOI: 10.3969/j.issn.2095-1191.2010.05.022
引用本文: 何奇松, 孙翔翔, 易春华, 蒋家霞, 付薇, 马琳, 熊毅. 2010: 白鹭源禽Ⅰ型副粘病毒L基因的克隆与序列分析. 南方农业学报, 41(5): 481-484. DOI: 10.3969/j.issn.2095-1191.2010.05.022
HE Qi-song, SUN Xiang-xiang, YI Chun-hua, JIANG Jia-xia, FU Wei, MA Lin, XIONG Yi. 2010: Cloning and sequence analysis of L gene of avian paramyxovirus 1 isolated from egret. Journal of Southern Agriculture, 41(5): 481-484. DOI: 10.3969/j.issn.2095-1191.2010.05.022
Citation: HE Qi-song, SUN Xiang-xiang, YI Chun-hua, JIANG Jia-xia, FU Wei, MA Lin, XIONG Yi. 2010: Cloning and sequence analysis of L gene of avian paramyxovirus 1 isolated from egret. Journal of Southern Agriculture, 41(5): 481-484. DOI: 10.3969/j.issn.2095-1191.2010.05.022

白鹭源禽Ⅰ型副粘病毒L基因的克隆与序列分析

Cloning and sequence analysis of L gene of avian paramyxovirus 1 isolated from egret

  • 摘要: 根据GenBank中已发表的鹅副粘病毒GPV-SF02株全基因组序列设计5对引物,采用RT-PCR扩增出白鹭源禽Ⅰ型副粘病毒(W13株)L基因的LA(1121 bp)、LB(1481 bp)、LC(1439 bp)、LD(1476 bp)、LE(1608 bp)5个片段,用DNA Star软件比铰分析后进行拼接,获得长约6760 bp包含有L基因全长的核苷酸序列,而W13株L基因的mRNA全长为6704 bp、开放阅读框(ORF)为6615个碱基、编码2204个氨基酸.氨基酸同源性分析表明:W13株L基因与鹅源SF02、ZJl、NA-1株及鸡源Guangxi7株的同源性均超过97.0%;而核苷酸同源性分析表明:W13株L基因与鹅源SF02、ZJl、NA-1株及鸡源Guangxi7株的同源性均超过93.0%.可见,W13株与鹅源SF02、ZJl、NA-1株及鸡源Guangxi7株的亲缘关系较近.

     

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