甘蔗常用亲本的田间抗旱指标筛选及抗旱性评价

Drought resistance index screening and drought resistance evaluation of commonly-used sugarcane parents in field

  • 摘要: 【目的】筛选甘蔗田间抗旱指标,并对甘蔗常用亲本进行抗旱性评价,为后续甘蔗种质资源抗旱鉴定和甘蔗抗旱评价标准制定提供理论依据,同时也为甘蔗抗旱新品种选育及抗旱机制研究提供种质保障。【方法】以20份甘蔗常用亲本为材料,包括3个国外引进亲本和17个国内亲本,设正常灌水对照和干旱胁迫处理,调查与抗旱性相关的农艺性状指标。采用显著性和相关分析筛选出与甘蔗抗旱性关系密切的指标,结合抗旱系数、隶属函数值、抗旱性综合评价值(D值)和聚类分析结果,综合评价甘蔗常用亲本的抗旱性。【结果】从48个农艺性状指标中,筛选出中度干旱时期绿叶率、重度干旱时期绿叶率、重度干旱时期株高、复水时期株高、第4节节间长、第4节茎径、第4节茎重和蔗茎产量8个适宜评价甘蔗抗旱性的指标;结合抗旱系数、隶属函数和D值评价了20份甘蔗常用亲本的抗旱性,由强到弱排序为桂糖31号(GT31)>桂糖32号(GT32)>新台糖22号(ROC22)>云蔗89-159(YZ89-159)>Ke5>粤糖00-236(YT00-236)>台糖172(F172)>CP72-1210>粤糖94-128(YT94-128)>柳城03-182(LC03-182)>福农39号(FN39)> CP80-1827 >粤糖93-159(YT93-159)>桂糖11号(GT11)>新台糖10号(ROC10)>桂糖29号(GT29)>新台糖16号(ROC16)>赣蔗18号(GZ18)>新台糖1号(ROC1)>桂糖21号(GT21)。通过聚类分析和田间缺水性状相结合,将20份甘蔗常用亲本的抗旱性分为五大类,其中CP72-1210、YT00-236、Ke5、ROC22、YZ89-159、GT31和GT32为高抗旱品种,F172、GT11、ROC1和ROC10为抗旱品种,LC03-182、FN39、CP80-1827、GT29和ROC16为中等抗旱品种,YT93-159为不抗旱品种,GZ18和GT21为极不抗旱品种。【结论】筛选获得8个适宜评价甘蔗抗旱性的指标可用于甘蔗种质资源抗旱鉴定和甘蔗抗旱评价标准制定。7个高抗旱品种可作为甘蔗抗旱性育种的重要亲本加以利用。

     

    Abstract: 【Objective】To screen drought resistance indicators for sugarcane in the field and evaluate drought resistance of commonly-used sugarcane parents,in order to provide theoretical basis for subsequent drought resistance identification of sugarcane germplasm resources and formulation of drought resistance evaluation standards,as well as to provide germplasm guarantee for breeding new drought-resistant sugarcane varieties and study on sugarcane drought resistance mechanism.【Method】Twenty commonly-used sugarcane parents,of which 3 were foreign and 17 domestic ones,were used as materials,two treatments of normal irrigation control and drought stress were set up to investigate agronomic trait indexes related to drought resistance. The indexes related to sugarcane drought resistance were selected by significance analysis and correlation analysis,and drought resistance of commonly-used sugarcane parents was comprehensively evaluated by drought resistance coefficient,subordinate function value,comprehensive evaluation value(D value)of drought resistance,and cluster analysis results.【Result】Among the 48 single agronomic traits indexes,8 indexes suitable for evaluating the drought resistance of sugarcane were selected,namely moderate drought period green leaf rate,severe drought period green leaf rate,severe drought period plant height,rewatering period plant height,length of the 4th node,diameter of the 4th node,weight of the 4th node and cane yield;the drought resistance of 20 commonly-used sugarcane parents was evaluated through drought resistance coefficient,subordinate function value and D value,and drought resistance was in the order of Guitang 31(GT31)>Guitang 32(GT32)>Xintaitang 22(ROC22)>Yunzhe 89-159(YZ89-159)>Ke5>Yuetang 00- 236(YT00-236)>Taitang 172(F172)>CP72-1210>Yuetang 94-128(YT94-128)>Liucheng 03-182(LC03-182)>Funong 39(FN39)>CP80-1827>Yuetang 93-159(YT93-159)>Guitang 11(GT11)>Xintaitang 10(ROC10)>Guitang 29(GT29)> Xintaitang 16(ROC16)>Ganzhe 18(GZ18)>Xintaitang 1(ROC1)>Guitang 21(GT21). Combining clustering analysis and field drought deficiency traits,the drought resistance was divided into 5 categories,among which CP72-1210,YT00- 236,Ke5,ROC22,YZ89-159,GT31 and GT32 were highly drought-resistant,while F172,GT11,ROC1 and ROC10 were drought-resistant,LC03-182,FN39,CP80-1827,GT29 and ROC16 were mediumly drought-resistant,and YT93-159 was not drought-resistant,and GZ18 and GT21 were extremely non-drought-resistant.【Conclusion】Eight indicators suitable for evaluating sugarcane drought resistance are screened and can be used for drought resistance identification of sugarcane germplasm resources and development of sugarcane drought resistance evaluation standards. Seven high drought-resistant varieties can be utilized as important parents for sugarcane drought resistance breeding.

     

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