花叶良姜离体再生体系的建立

Establishment of in vitro propagation technology of Alpinia zerumbet Variegata

    摘要
  • 摘要: 【目的】建立花叶良姜高频高效离体再生体系,为其种苗周年规模化生产提供新方法。【方法】以花叶良姜种子为材料,开展无菌萌发、愈伤组织诱导、愈伤组织分化及生根培养研究。【结果】花叶良姜种子依次经75%乙醇处理60 s、0.1%升汞处理10 min、5%次氯酸钠处理3 min,污染率仅5.00%,萌发率为75.83%;适宜花叶良姜愈伤组织诱导的培养基为MS+1.50 mg/L 6-BA+0.30 mg/L 2,4-D,适宜花叶良姜愈伤组织分化的培养基为MS+2.00 mg/L TDZ+0.10 mg/L 2,4-D,分化系数达10.03;花叶良姜组培苗最佳生根培养基为1/2MS+1.00 mg/L ABT1号生根粉,生根率为98.00%。【结论】通过愈伤组织途径可建立花叶良姜的离体再生体系。

     

    Abstract: ObjectiveThe present study was conducted to establish high-efficiency and high-frequency in vitro re-generation system of Alpinia zerumbet Variegata,in order to provide a new method for annual large-scale production of A. zerumbet seedling. MethodUsing A. zerumbet seeds as materials,the experiment on aseptic budding,callus induction, callus differentiation and rooting was carried out. ResultResults showed that, after A. zerumbet seeds were disinfected with 75%ethanol for 60 s,then 0.1%mercury chloride for 10 min,finally 5%sodium hypochlorite for 3 min,contamination rate was only 5.00%,and germination rate was 75.83%. Suitable medium for inducing callus was MS+1.50 mg/L 6-BA+0.30 mg/L 2,4-D,suitable medium for callus differentiation was MS+2.00 mg/L TDZ+0.10 mg/L 2,4-D,differentiation coef-ficient was up to 10.03. Furthermore,optimum medium for rooting was 1/2MS+1.00 mg/L ABT1 rooting powder,rooting rate was 98.00%.ConclusionIn vitro regeneration system of A. zerumbet can be established by the way of callus culture.

     

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