Abstract: ObjectiveThe present study was conducted to screen in vitro propagation condition for Vaccinium spp. Sunshine Blue in order to establish a rapid propagation system for blueberry Sunshine Blue. MethodIn this experiment, using Sunshine Blue’s aseptic seedling stems as explants, proper medium, initial shoot number, cytokinins, auxins, pH value and activated carbon(AC) content which were suitable for shoot proliferation and rooting were screened. ResultAt shoot proliferation stage,the stems cultured in WPM medium had the largest number of proliferation shoots and the average shoot length was long after 60 days of culture. Therefore, WPM medium could be used as a proper medium for stem prolif-eration. Inoculating the stems with two initial shoots into WPM+2.0 mg/L ZT+0.01 mg/L IBA medium, the number of pro-liferation shoots was 10.25 after 30 days of culture, significantly more than those in other media(P<0.05); the number reached 17.43 after 60 days of culture, which was more than those in other media. At rooting stage,the stems cultured in medium with 0.10 mg/L IBA auxin, 1000.0 mg/L activated carbon realized 87.5% rooting rate after 90 days of culture, which was higher than those cultured in other media. ConclusionIn terms of in vitro propagation of Vaccinium spp. Sun-shine Blue stems with two initial shoots, the optimal medium for shoot proliferation is WPM+2.0 mg/L ZT+0.01 mg/L IBA, the most appropriate rooting medium is 1/2WPM+0.10 mg/L IBA+1000.0 mg/L AC.