Abstract: ObjectiveThe present study was conducted to compare anatomical structural differences among different Citrus reticulata Blanco cv. Kinokuni varieties(lines) and evaluate their cold resistance and drought resistance, in order to provide references for variety introduction and regional distribution of Citrus reticulata Blanco cv. Kinokuni. MethodSec-tion was made according to paraffin section making process and put under fluorescence microscope. Mature leaves anatom-ical structures of seven varieties(lines) of C. reticulata Blanco cv. Kinokuni planted in Guangxi Academy of Specialty Crops resources garden were observed and compared. Then cold resistance and drought resistance were evaluated by CTR and membership function. ResultThe leaf thickness of Miguangju,Liuchengmiju,Guiju No.1,Nanfeng No.1,Early Nanfeng-miju,Nanfeng No. 2 and Yangxiao 2-6 were 196.53-255.76μm,upper epidermis thickness 15.04-18.09μm, palisade tis-sue thickness 32.37-42.88 μm, spongy tissue thickness 131.55-181.05 μm,lower epidermis thickness 13.08-15.77μm, cell tense ration (CTR) of leaf 16.60-19.43. The order of cold resistance from strong to weak was as follows: Nanfeng No.2>Nanfeng No.1>Early Nanfengmiju>Liuchengmiju>Guiju No.1>Miguangju>Yangxiao 2-6. The order of drought resistance from strong to weak were as follows:Liuchengmiju>Nanfeng No.1>Miguangju>Nanfeng No.2>Early Nanfengmiju>Guiju No. 1>Yangxiao 2-6. ConclusionNanfeng No.2, Nanfeng No.1, Liuchengmiju and Early Nanfengmiju are more cold-resistant and more drought-resistant than Guiju No.1 and Yangxiao 2-6. Miguangju is drought-tolerated but not cold-resis-tant. In actual production, C. reticulata Blanco cv. Kinokuni variety introduction and promotion should match the topogra-phy and climate in the regions, and formulate reasonable cultivation and management measures.