Abstract: ObjectiveThe detection situation of main virulence genes in quinolones-resistant pathogenic Aeromonas hydrophila and resulting mutation of drug-resistant gene and in China, in order to provide reference for study on control of A. hydrophila and its virulence genes and quinolone-resistant molecular mechanisms. MethodThe reports on quinolones-re sistant molecular mechanisms, virulence genes and pathogenesis of A. hydrophila was collected by computer-based online search of CNKI, WANFANG DATA, VIP in Chinese science and technology periodical database and Duxiu Knowledge Base(from set-ting-up database to April 2016). Then Meta analysis was performed by Review Manager 5.3 published by Cochrane Collabora-tion network, and indirect comparative Meta analysis was performed by ITC published by Canadian Health Pharmaceutical Technology Agency. ResultA total of 31 literatures were included, 19 of which detected virulence genes of pathogenic strains (457 strains), 11 of which detected drug-resistant genes of pathogenic strains(101 strains), 811 of which detected mutational site of drug-resistant gene in drug-resistant strains(88 strains). The detection rates of virulence genes of pathogenic A. hy-drophila in China were as follows: astA 91.30%, altA 80.42%, aerA 72.77%, hlyA 66.85%, actA 62.13%, ahpA 56.18%, ahaI 53.04%. North of the Huaihe River was dominated by hlyA gene, with detection rates of 67.31%, and significantly higher than the south of Huaihe River(P<0.05, the same below), and higher than the national average detection rate. South of the Huaihe Riv-er was dominated by actA gene, with detection rate of 93.59%, and significantly higher than north of the Huaihe River area, and higher than the national average detection rate too. The detection rates of plasmid-mediated drug-resistant genes of pathogenic A. hydrophila in China were as follows: qnrB 50.00%, qepA 32.00%, qnrS 27.91%, qnrA 6.98%, but qnrC and qnrD were undetected. The detection rate of single site mutation of gyrA83 site was significantly higher than that of double sites mutations of gyrA83 and parC87OR=0.49, 95% CI(0.08, 3.09), P=0.008. ConclusionThe detection method of pathogenic A. hydrophila in China: actA and aerA are taken as judgment standard of strong pathogenicity in south of the Huaihe River; hlyA and aerA are taken as judgment standard of strong pathogenicity in north of the Huaihe River. At present, mutational site of gene is mainly gyrA83 single site mutation and gyrA83, parC87 double sites mutations in quinolones-resistant A. hydrophila in China.