上饶早梨主栽品种离体快繁体系的建立
Establishment of in vitro rapid propagation system of main cultivars of Shangrao early pear
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摘要: 【目的】建立上饶早梨主栽品种六月雪和黄皮消的离体快繁技术体系,为上饶早梨试管苗的快速繁殖提供参考依据。【方法】对六月雪和黄皮消进行组织培养,筛选适宜的外植体、灭菌方式、培养基和移栽基质。【结果】六月雪和黄皮消理想的外植体为长7.0~8.0 cm的新生幼嫩带芽茎段,理想的灭菌方式为75%酒精消毒1.0 min+0.1%氯化汞消毒10~12 min,理想的腋芽诱导培养基为MS+2.0 mg/L 6-BA+0.2 mg/L IBA,理想的不定芽增殖培养基为MS+1.0 mg/L KT+0.2 mg/L NAA,繁殖系数为3.6~3.8,理想的不定芽生根培养基为1/2MS+0.3 mg/L NAA+0.3 mg/L PP333+0.5 g/L AC(活性炭),理想的试管苗移栽基质为泥炭∶珍珠岩∶蛭石=4∶1∶1或腐殖土∶珍珠岩=5∶1,移栽后浇灌含0.5 mg/L PP333的MS基本培养液可显著提高试管苗成活率(P<0.05),驯化移栽成活率在90.0%以上。【结论】建立的六月雪和黄皮消离体快繁体系,可供上饶早梨组培苗工厂化生产参考利用。Abstract: ObjectiveThe present study was conducted to establish in vitro rapid propagation system of main cultivars of Shangrao early pear namely Liuyuexue and Huangpixiao, in order to provide reference for rapid propagation of test-tube plantlet of Shangrao early pear. MethodTissue culture of Liuyuexue and Huangpixiao was conducted, suitable explants, disinfection method, culture medium and transplanting matrices were selected. ResultResults showed that ideal explant of Liuyuexue and Huangpixiao was new young stems with buds of 7.0-8.0 cm in length; ideal disinfection method was 75% alcohol for 1.0 min+0.1% mercuric chloride for 10-12 min; ideal axillary bud induction medium was MS+2.0 mg/L 6-BA+0.2 mg/L IBA; ideal adventitious bud proliferation culture medium was MS+1.0 mg/L KT+0.2 mg/L NAA with coef-ficient being 3.6-3.8; ideal adventitious buds rooting culture medium was 1/2MS+0.3 mg/L NAA+0.3 mg/L PP333+0.5 g/L AC(active carbon), ideal transplanting matrix of plantlets was peat∶perlite∶vermiculite=4∶1∶1 or humus soil∶perlite=5∶1. After transplanting, watering MS culture fluid containing 0.5 mg/L PP333 could significantly improve survival rate of plantlets(P<0.05),which was up to 90.0%. ConclusionThe established in vitro rapid propagation system of Liuyuexue and Huang-pixiao can provide reference for factory production of tissue culture seedling of Shangrao early pear.
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