Abstract: ObjectivePropagation system established with sterile leaves of Oriental lily Love Story as material to provide technical reference for industrialized production of its seed-bulbs. MethodSterile scales and leaves from flower organs culture were used as material and MS was used as basic medium, adding different concentrations of 6-BA and 2,4-D to induce adventitious buds and bulb-lets; using different concentrations of sugar to induce bulb-lets expansion and rooting; perlite, peat and vermiculite were mixed as the transplanted matrix of bulb-lets. ResultMS+1.5 mg/L 6-BA was appropriate for inducing adventitious buds with sterile scales and induction rate was 75.0% ; the suitable sterile leaves differentiation bulb -lets medium was MS +2.0 mg/L 2,4-D and induction rate was 91.7% ; the MS medium added 70.0 g/L sugar was suitable to induce bulb-lets expansion and rooting; perlite ∶peat∶vermiculite=1∶1∶1 was the best transplanted matrix of plant-lets with bulb-lets and roots, transplanting survival rate got 100.0% in this matrix.ConclusionOriental lily Love Story could be established asexually rapid propagation system by sterile leaf.