Abstract: ObjectiveThis research explored sequence and expression of calcineurin catalytic subunit in Volvariella volvacea(Vv-CNA), in order to provide reference for growth and development of V. volvacea and reveal the regulatory me-chanism of fruit bodies. MethodThe sequence and conserved structure of Vv-CNA were analysed by bioinformatics. An-ticipated V. Volvacea CNA amino acid sequence by ORF-finder was compared with CNA amino acid sequences of other fu-gus downloaded from NCBI. Fluorescent quantitative PCR was used to detect relative expression of Vv-CNA at different growth stages of V. volvacea. ResultThe coding region of Vv-CNA was 2503 bp, including 10 introns(intron retention was detected in I2, I8, I9 and I10). It encoded 610 amino acids and GenBank accession number was KX463514. Homolo-gous analysis was conducted between Vv-CNA amino acid sequence and amino acid sequences of animal(human, mouse), plant(arabidopsis, rice) and fungus(aspergillus, yeast). The results indicated that Vv-CNA contained three conserved do-mains, which were most closely related to Gloeophyllum Trabeum. Vv-CNA upregulated at egg stage and elongation stage of fruit body, and reached the peak at egg stage. ConclusionVv-CNA gene encodes calcineurin catalytic subunit and its up-regulated expression may be related to elongation of stipe in V. volvacea.