摘要:
[目的]优化超声波辅助酶解马氏珠母贝肉工艺条件,并对酶解产物进行自由基清除活性分析,为马氏珠母贝肉的高值化利用提供参考依据.[方法]以马氏珠母贝肉为原料,采用单因素及响应面试验分析酶解温度、加酶量、超声功率和酶解时间对珠母贝肉蛋白质水解度的影响,确定最佳工艺参数,并测定酶解产物对羟基自由基(·OH)、超氧自由基(O2)及DPPH自由基(DPPH·)的清除能力.[结果]影响马氏珠母贝肉酶解效果的4个因素主次顺序为:超声波功率>加酶量>酶解温度>酶解时间,其中酶解温度、加酶量和超声波功率影响极显著(P<0.0 1),酶解时间及加酶量与超声波功率的交互作用影响显著(P<0.05).马氏珠母贝肉最佳超声波辅助酶解条件为:酶解温度48℃、加酶量2200 U/g'超声波功率13%(总输出功率900W)、酶解时间12 min,在此条件下的蛋白质水解度为21.937%,与预测值(22.140%)的相对误差为0.92%.酶解产物对·OH、O2-和DPPH·的清除能力随酶解产物质量浓度的增加而增强,当质量浓度为2.0 mg/mL时,清除率分别为68.85%、83.62%和82.36%.[结论]优化得到的超声波辅助酶解马氏珠母贝肉工艺参数准确可靠,酶解时间显著缩短,酶解产物具有明显的清除自由基活性,可作为具有抗氧化作用的保健食品、保健酒等产品的优质原料.
Abstract:
[Objective]The present study optimized ultrasonic-assisted enzymolysis conditions for Pinctada fuctada martensii muscle,and analyzed free radical scavenging activity of enzymatic hydrolysate,in order to provide reference for value-added utilization of P.fuctada martensii muscle.[Method] P.fuctada martensii muscle was taken as the material,single factor and response surface methodology were applied to analyze effects of enzymolysis temperature,protease dosage,ultrasonic power and enzymolysis time on degree of hydrolysis(DH) of protein in P.fuctada martensii muscle,and determine the optimum process parameters,and measure scavenging activity of enzymatic hydrolysate on hydroxyl radical (· OH),superoxide radical(O2) and DPPH free radical(DPPH·).[Result]The factors influencing enzymolysis of P.fuctada martensii muscle in order of their effects were as follows:ultrasonic power>protease dosage>enzymolysis temperature>enzymolysis time.Enzymolysis temperature,protease dosage and ultrasonic power had extremely significant difference on enzymolysis(P<0.01),and interaction between ultrasonic power and enzymolysis time had significant difference (P<0.05).The optimal enzymolysis condition was as follows:enzymolysis temperature was 48 ℃,protease dosage 2200 U/g,ultrasonic power 13% (total output power was 900 W),enzymolysis time 12 min.Under such condition,DH of protein was 21.937%,the relative error to expected value(22.140%) was 0.92%.The scavenging activity of enzymatic hydrolysate on · OH,O~ and DPPH· enhanced as mass concentration of enzymatic hydrolysate increased.When the mass concentration of enzymatic hydrolysate reached 2.0 mg/mL,the scavenging rate for ·OH was 68.85%,scavenging rate for O2-83.62% and scavenging rate for DPPH· 82.36%.[Conclusion]The optimized process parameters of ultrasound-assisted enzymolysis are accurate and reliable.Enzymolysis time is shortened and free radical scavenging activity of enzymatic hydrolysate is obvious.Therefore,the enzymatic hydrolysate can be utilized as raw materials of health care food,health care drinks with antioxidation.
百度学术
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