Abstract: ObjectiveThe present study improved preparation conditions for black fungus serous liquid to provide the basis for deep processing of black fungus and its application.MethodDry fruiting body and fermented mycelium of selenium-enriched black fungus G6 were taken as the materials.They were shattered and added distilled water according to certain ratios.Contents of insoluble material,selenium,crude protein,soluble sugar,crude fat,crude fiber and their dissolution rates were measured in order to determine the optimum material-water ratio,and made fruiting body serous liquid and mycelium serous liquid.Orthogonal experiment was adopted to optimize preparation conditions for serous liquid of selenium-enriched black fungus.ResultInsoluble material content in fruiting body serous liquid was higher than that in fermented mycelium serous liquid;nutrient contents in fermented mycelium serous liquid were higher thau those in fruiting body serous liquid.As material-water ratio decreased,all nutrient contents in both serous liquids dropped,but their dissolution rates increased.The optimal material-water ratio for fermented mycelium serous liquid was 1∶10,and 1∶20 for fruiting body serous liquid.Mixing 300 mL mycelium serous liquid,200 mL fruiting body serous liquid and 30 g honey (25 mL)into black fungus serous liquid.The taste was smooth,slightly sweet and had light fragrance of black fungus.After three month,the black fungus serous liquid did not layer and maintained stable.ConclusionThe optimal ratio for black fungus serous liquid is fruiting body serous liquid:mycelium serous liquid∶honey being 8∶12∶1.The drink is dark brown and sticky.The texture and flavor are good.There is no need to add chemical thickener in it.It is a kind of green drink with high nutrient value.