基于主成分分析与隶属函数法的三角梅种质资源综合评价

Comprehensive evaluation of Bougainvillea germplasm resources based on principal component analysis and membership function method

  • 摘要:目的】对金沙江干热河谷三角梅种质资源进行综合评价,为干热河谷地区优良三角梅品种筛选及新品种选育提供理论依据。【方法】以18份三角梅种质为研究对象,测定11个品种性状及5种色素含量指标,并进行群众评分及花色分组,通过变异系数(CV)分析、聚类分析、相关分析、主成分分析(PCA)及隶属函数分析等方法对三角梅种质进行综合评价。【结果】18份三角梅种质按花苞颜色可分为紫色组、红色组、橙黄色组、粉色组、白色组,其数量性状的变异系数介于28.05%~39.93%,其中叶片宽变异系数最大(39.93%),苞片宽变异系数最小(28.05%);色素含量的变异系数介于26.52%~46.62%,其中原花青素含量的变异系数最大(46.62%),叶绿素a含量的变异系数最小(26.52%)。相关分析结果显示,原花青素含量与甜菜红素含量和花色均呈极显著负相关(P<0.001),且相关系数均为-0.87;花色与甜菜红素含量呈极显著正相关(P<0.001),相关系数为0.88;群众评分与花色、叶片长、原花青素含量和甜菜红素含量均存在显著(P<0.05)或极显著(P<0.01)相关性,其中与花色的相关性最强。聚类分析结果显示,18份三角梅种质被划分成四大类群,其中第二类群仅含马尼拉小姐,说明马尼拉小姐与其他种质相比更具独特性;11个品种性状和5种色素含量被划分为四大类,其中第一类和第二类主要体现性状特征,第三类和第四类主要体现色素含量特征。PCA分析以特征值大于1为提取标准,共得到第一主成分~第六主成分等6个主成分,累计贡献率为82.85%,6个主成分中因子载荷最高的性状分别为花色、苞片宽、叶片长、叶片宽、叶片颜色和叶绿素a含量。通过计算隶属函数值,发现基利耶坎普贝尔的得分最高。综合PCA分析与隶属函数法计算综合得分,将18份三角梅种质划分为四个等级,其中I级包括马尼拉小姐、巴特夫人、基利耶坎普贝尔。【结论】结合PCA分析和隶属函数法进行综合评价,筛选到马尼拉小姐、巴特夫人、基利耶坎普贝尔等3份综合得分较高的三角梅种质资源,其花苞和叶片面积大、花苞颜色鲜艳,适宜在金沙江干热河谷地区推广种植。

     

    Abstract:Objective】This study aimed to comprehensively evaluate Bougainvillea germplasm resources in the dry-hot valley of the Jinsha River, providing theoretical reference for superior variety selection and new variety breeding of Bougainvillea in the dry-hot valley of the Jinsha River.【Method】A total of 18 Bougainvillea germplasms were taken as research objects to measure 11 cultivar traits and five pigment content indicators. Public scoring and grouping by color category were performed. Comprehensive evaluation of Bougainvillea germplasms was conducted based on analysis of coefficient of variation (CV), cluster analysis, correlation analysis, principal component analysis (PCA), and membership function analysis.【Result】The 18 Bougainvillea germplasms were divided into purple group, red group, orange-yellow group, pink group, and white group,and the CV of quantitative traits ranged from 28.05%-39.93%, with the largest CV for leaf width at 39.93% and the smallest CV for bract width at 28.05%. The CV of pigment content ranged from 26.52% to 46.62%, with the largest CV for procyanidin content at 46.62% and the smallest CV for chlorophyll a content at 26.52%. Correlation analysis results showed that the procyanidin content had extremely significant negative correlations with betacyanin content and flower color (P<0.001), with the coefficient of correlation of -0.87; flower color had an extremely positive correlation with betacyanin content (P<0.001), with the coefficient of correlation of 0.88; public scoring had significant (P<0.05) or extremely signficant (P<0.01) correlations with flower color, leaf length, procyanidin content, and betacyanin content, with the most pronounced correlation with flower color. Cluster analysis results showed that the 18 germplasms into four major groups, among which Group Ⅱ only included Bougainvillea cv. Miss Manila, demon-strating that this cultivar showed greater uniqueness compared with other germplasms; the 11 cultivar traits and five pigment content indicators were divided into four major categories: the first and second categories showed trait characteristics, and the third and fourth categories showed pigment content characteristics. Principal component analysis(PCA) showed that, six principal components from the first to the sixth principal component were extracted at the standard of eigenvalue greater than 1, with the cumulative contribution rate of 82.85%, and the traits that exhibited the highest factor loadings across the six principal components (PC1-PC6) were flower color, bract width, leaf length, leaf width, leaf color, and chlorophyll a content. By calculating the membership function values, Bougainvillea cv. Killie Campbell had the highest score. According to calculation combined comprehensive PCA with membership function method, the 18 Bougainvillea germplasms were divided into four grades, and the Grade I included Bougainvillea cv. Miss Manila, Bougainvillea cv. Mrs. Butt, and Bougainvillea cv. Killie Campbell.【Conclusion】According to comprehensive evaluation of PCA and membership function method, three Bougainvillea cultivars (Bougainvillea cv. Miss Manila, Bougainvillea cv. Mrs. Butt, and Bougainvillea cv. Killie Campbell) have high scores. As they have big bracts, big leaves, and bright bracts, they are suitable to be popularized and cultivated in the dry-hot valley of the Jinsha River.

     

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