Abstract: 【Objective】 To characterize the growth conditions and whole genome sequencing data of the endophytic fungus Paraphaeosphaeria sporulosa isolated from Dracaena cambodiana Pierre ex Gagn.，and to experimentally validate its resin induction potential， which could provide reference for the interaction mechanisms between Dracaena cambo-diana Pierre ex Gagn. and endophytic fungus，as well as for supporting the development and utilization of endophytic fungus-derived resources for dragon’s blood resin.【Method】 The strain Paraphaeosphaeria sporulosa was isolated and its growth conditions under different growth conditions were investigated. The optimal growth parameters， including temperature， pH， light cycle， carbon sources， and nitrogen sources， were screened. Genome sequencing and assembly were performed for Paraphaeosphaeria sporulosa. Gene functions were annotated using GO，KEGG，KOG，CAZY，and anti-SMASH databases to identify potential pathogenicity-related factors. Through experiments on inoculating the Paraphaeosphaeria sporulosa strain onto Dracaena cambodiana Pierre ex Gagn.， the inducing effect of this strain on resin secretion was verified. 【Result】 The hyphae of Paraphaeosphaeria sporulosa exhibited a smooth surface and consisted of transpa-rent，seperated hyphae structure. The hyphae were highly branched，radially expanding and forming an interconnected network-like structure. The optimal growth conditions were 25 ℃，weakly acidic conditions （pH=5），full light exposure，with starch or sucrose as carbon sources，and sodium nitrate or ammonium chloride as nitrogen sources. The genome size of Paraphaeosphaeria sporulosa was 39689644 bp. A total of 2041 and 1163 genomes were annotated to membrane- and nucleus-related GO terms respectively， suggesting that cellular membrane and nuclear functions played important roles in environmental adaptation. KEGG analysis showed that 71.60% of genes were enriched in metabolic pathways，while 14.70% were enriched in genetic information processing. KOG database revealed that genes annotated in signal transduction mechanism represented the largest functional category，with 214 genes. A total of 684 genes were annotated in the CAZY database，of which 328 were annotated in carbohydrate esterase family （GHs）. In antiSMASH database， 79 genes annotated in 6 types of secondary metabolite. biosynthetic pathways. The virulence factor gene G00129750.1 was screened， which encoded a protein containing FKBP-C domain. After inoculating Paraphaeosphaeria sporulosa， the resin secretion of Dracaena cambodiana Pierre ex Gagn. was greatly increased compared to CK， and the resin was widely distributed with a deep color. 【Conclusion】 Paraphaeosphaeria sporulosa can interact with Dracaena cambodiana Pierre ex Gagn. and may promote resin secretion via a grey-mold-like infection mechanism.