Abstract: 【Objective】 To identify acyl-CoA oxidase （VsACX） gene family members in Vitis vinifera ssp. sylvestris and analyze their expression patterns， so as to provide theoretical reference for further elucidating the molecular mechanisms by which VsACX gene family regulated growth and stress adaptation in Vitis vinifera ssp. sylvestris， as well as breeding new grape varieties with stress resistance. 【Method】 Based on the genomic data of Vitis vinifera ssp. sylvestris VS-1， members of VsACX gene family were identified. Bioinformatics tools were utilized to predict and analyze the physicochemical properties of proteins， subcellular localization， phylogenetic relationships， protein secondary structures， conserved motifs， and cis-acting elements. Transcriptome data from various tissues and organs of grape at different deve-lopmental stages were compared to characterize the expression patterns of VsACX gene family. Two-year-old cultivated Eurasian grape variety Chardonnay was subjected to mechanical damage and 200 μmol/L methyl jasmonate （MeJA） stress treatments. Real-time fluorescence quantitative PCR was performed to detect the relative expression of VsACX family genes at different treatment times （0， 2， 4， and 6 h）. 【Result】 Four VsACX gene family members， namely VsACX1， VsACX2， VsACX3， and VsACX4， were identified in Vitis vinifera ssp. sylvestris， which were distributed on chromosomes 2， 7， 8， and 12 respectively， and all predicted to localize on peroxisomes. VsACX1， VsACX2， and VsACX3 genes exhibited one-to-one collinear relationships in the genomes of Pinot Noir grape and tomato. The number of introns varied among different VSACX family members， ranging from 4 to 13. Motif 3 and Motif 2 were exclusively detected in VsACX3 and VsACX4 proteins， and all VsACX famly proteins harbored highly conserved ACOX superfamily domain. The secondary structures of VsACX proteins were predominantly composed of α-helixes. Phylogenetic analysis classified the VsACX family proteins into three subgroups. A total of 27 cis-acting elements falling into three categories （growth- and development-related response， hormone response ， and stress response） were identified in the promoter regions of VsACX family genes. VsACX family genes displayed tissue-specific expression patterns： VsACX2 gene showed the highest expression in stamens and seedlings， while VsACX3 and VsACX4 genes were more highly expressed in most reproductive organs than in vegetative organs. Upon 3 h of mechanical damage， the relative expression of VsACX1 and VsACX2 genes increased extremely significantly compared with the 0 h （P<0.01）. Under MeJA treatment for 0-6 h， VsACX1， VsACX3， and VsACX4 genes presented a biphasic expression pattern characterized by an initial decrease followed by a subsequent increase. 【Conclusion】 Four VsACX gene family members are identified. These members are highly conserved in evolution yet exhibit obvious functional divergence and tissue-specific expression， and their expression levels respond to mechanical damage and MeJA treatments.