开花期低温胁迫对优质香稻产量和品质的影响

Effects of low temperature stress at flowering stage on yield and quality of high-quality fragrant rice

  • 摘要: 【目的】 探究开花期低温胁迫对香稻抽穗期至成熟期干物质生产、产量和品质的影响,为抗寒露风冷害香稻品种选育提供理论参考。【方法】 采用盆栽方式和二因素完全随机试验设计,设香稻品种和低温处理时间2个因素。其中,品种设2个水平,分别为河苗真香(耐冷性强)和河丽雅香(耐冷性弱);低温处理时间设4个水平,分别为开花期日平均温度20.5 ℃持续0、3、6和9 d,以0 d为对照(CK)。水稻抽穗期至成熟期,测定茎鞘物质输出率和转化率、群体生长率、净同化率、叶面积衰减率;水稻成熟期测定产量及其构成因素;收获保存1个月后测定加工品质、外观品质及化学和食味品质,分析开花期低温胁迫对香稻产量和品质的影响,并对不同指标进行相关分析。【结果】开花期低温9 d处理显著降低2个香稻品种的实粒数、结实率、抽穗期至成熟期的叶面积衰减率及河苗真香的茎鞘物质输出率(P<0.05,下同),导致产量下降。开花期低温6 d处理显著降低了2个香稻品种的整精米率,降低了粒宽,提高了粒长宽比。开花期低温6和3 d处理分别显著降低了河苗真香和河丽雅香的垩白度。开花期低温3、6和9 d处理均显著提高2个香稻品种的蛋白质含量,低温9 d处理显著降低了河苗真香的食味值。相关分析结果表明,河苗真香的产量与实粒数呈极显著正相关(P<0.001),与净同化率和叶面积衰减率呈显著正相关;其食味值与糙米率和蛋白质含量呈显著负相关,与整精米率呈显著正相关。河丽雅香的产量与实粒数、结实率、群体生长率、净同化率和叶面积衰减率呈显著正相关;其食味值与糙米率呈极显著负相关(P<0.01)。【结论】开花期低温降低了2个香稻品种抽穗期至成熟期的叶面积衰减率、茎鞘物质输出率、实粒数和结实率,导致香稻减产;低温还降低了整精米率和大米粒宽,增加了大米蛋白质含量,降低了食味值,使香稻品质变劣。

     

    Abstract: 【Objective】 This study aimed to investigate the effects of low temperature stress at flowering stage on dry matter production, yield, and quality from heading to maturity in fragrant rice, which could provide physiological basis for breeding cultivars against cold damage during the cold dew wind period. 【Method】 A two-factor completely rando-mized experimental design was adopted, with pot culture as the cultivation method. The two factors were fragrant rice cultivar and low temperature. Two levels were set for cultivar: Hemiaozhenxiang (with strong cold resistance) and Heliya-xiang (with low cold resistance). Four levels of low-temperature treatment time were established, namely 0, 3, 6, and 9 d of continuous exposure to the daily mean temperature of 20.5 ℃ at flowering stage, with 0 d treatment used as the control (CK). Measurements were taken for export rate and translocation rate of stem-sheath dry matter, crop growth rate, net assimilation rate, leaf area decreasing rate from rice heading to maturity. The yield and its components were assessed at maturity. Following one month of post-harvest storage, milling quality, appearance quality, chemical and taste quality were determined. The effects of low temperature stress at the flowering stage on the yield and quality of fragrant rice was examined, along with correlation analysis on various parameters. 【Result】 Low temperature for 9 d at flowering stage significantly reduced the leaf area decreasing rate, filled grain number, seed setting rate of Hemiaozhenxiang and Heliya-xiang, and the stem-sheath matter export rate of Hemiaozhenxiang from heading to maturity(P<0.05,the same below), leading to a decrease in yield. Low temperature for 6 d at flowering stage all significantly reduced the head rice rate, and grain width, but significantly increased the grain length-to-width ratio in both fragrant rice cultivars. Low temperature for 6 and 3 d at flowering stage significantly reduced the chalkiness degree in Hemiaozhenxiang and Heliyaxiang respectively. Low temperature for 3, 6, and 9 d at flowering stage all significantly increased the protein content in both cultivars, and 9 d of low temperature treatment significantly reduced the taste score of Heliyaxiang. Correlation analysis showed that the yield of Hemiaozhenxiang was extremely significantly and positively correlated with filled grain number (P<0.001),and was significantly and positively correlated with net assimilation rate, and leaf area decreasing rate; its taste score was significantly and negatively correlated with brown rice rate and protein content, significantly and positively correlated with head rice rate. The yield of Heliyaxiang was significantly and positively correlated with filled grain number, seed setting rate, crop growth rate, net assimilation rate, and leaf area decreasing rate: its taste score was extremely significantly and negatively correlated with brown rice rate (P<0.01). 【Conclusion】 Low temperature at flowering stage reduces leaf area decreasing rate, stem-sheath matter export rate from heading to maturity, filled grain number, and seed setting rate, lea-ding to yield loss in fragrant rice; low temperature reduces head milled rice rate and grain width, increases protein content, and decreases taste score, thereby deteriorating fragrant rice quality.

     

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