Abstract: 【Objective】 This study aimed to analyze the effects of different plant ash addition amounts on soil fungal community characteristics of morel， providing theoretical basis for appropriate application in morel cultivation. 【Method】 Taking Morchella sextelata as the experimental material， and morel soil samples at primordium period （40 d after sowing） with plant ash addition amounts of 0 （no addition）， 1%， 3%， and 5% were collected. The Illumina NextSeq PE300 high-throughput sequencing technology combined with multivariate statistical analysis method was used to analyze the relative abundance， diversity， inter-group differences， functional prediction， correlation between microbial communities and physicochemical properties， and so on. Characteristics of microbial community structure and functions were revealed. 【Result】 Under the three treatments of different plant ash addition amounts， physicochemical properties and fungal community structure of soil changed significantly （P<0.05， the same below）compared with those without plant ash addition. With the increase of plant ash addition amount， the soil pH and available potassium content increased significantly， while exchangeable calcium content decreased significantly. The plant ash addition amounts and nitrogen conversion showed a threshold effect： at the 0-3% addition amount， the nitrate nitrogen accumulation was promoted， but at the 5% addition amount， the nitrate nitrogen content decreased compared to that at the 3% addition amount， with the most significant difference in soil fungal community at the 5% addition amount. Functional prediction showed that the excessive plant ash addition led to the change in fungal nutritional type， and the most pathological nutritional types were observed at the 5% addition amount. Correlation analysis results between physicochemical properties and microbial community showed that the structural reorganization of fungal communities under the treatment of the 5% addition amount was related to microbiota imbalance. In addition， beneficial fungi such as Mortierellomycota and Mortierella increased at the 1% and 3% addition amounts， but decreased at the 5% addition amount. Mortierella showed an extremely significant positive correlation with exchangeable calcium content （P<0.01）， with the relative abundance of Penicillium and Aspergillus showing positive correlation with contents of ammonium nitrogen and exchangeable calcium. 【Conclusion】 Plant ash addition can markedly alter the soil physicochemical properties of morel， thereby reshaping the soil fungal community structure. Adding an appropriate plant ash amount can promote the growth of beneficial fungi and inhibit the growth of pathogenic fungi； however， excessive plant ash addition can lead to microbiota imbalance， adversely affecting morel. Plant ash addition may cause calcium ion loss， so， plant ash addition at a gradient of 1%-3% and calcium sources supplementation such as gypsum are recommended in practice to synergistically promote morel growth.