钾肥和腐殖酸互作对核桃果实糖代谢酶及品质的影响

Effects of potassium fertilizer and humic acid interactions on glycometabolic enzymes and quality of walnut fruits

  • 摘要: 【目的】 采用多级模糊综合评价(MFCE)方法探究钾肥和腐殖酸互作对核桃果实糖代谢酶及品质的影响,以期为新疆核桃生产园的合理施肥提供理论依据。【方法】 以温185核桃为试验材料,采用随机区组试验设计,设4个钾肥(K)水平,分别为0 kg/株(K0)、0.3 kg/株(K1)、0.6 kg/株(K2)和0.9 kg/株(K3),以及4个腐殖酸(H)水平,分别为0 kg/株(H0)、0.05 kg/株(H1)、0.075 kg/株(H2)和0.1 kg/株(H3),共16个处理。测定核桃果实外观和内在品质指标、糖组分含量和糖代谢酶活性,并采用MFCE方法构建核桃果实品质的综合评价模型,得出综合指数(CI),分析钾肥和腐殖酸互作对核桃果实品质的影响。【结果】 极差分析结果显示,K0水平的纵径和侧径均最大,K1水平的单果重最重,K2水平的糖代谢酶活性以及糖组分和可溶性糖含量均最高,K3水平的粗蛋白含量最高、横径最大;H1水平的蔗糖含量最高,H2水平的糖代谢酶活性以及果糖、葡萄糖、淀粉和可溶性糖含量均最高,H3水平的粗脂肪和粗蛋白含量最高、横径和侧径最大、单果重最重。综合评价结果显示,T8处理的CI最高,为5.062;其次是T5处理,为4.733;T16处理的CI最低,为0.472。回归分析结果显示,钾肥施用量和CI之间回归关系显著(P<0.05),其最高施用量为0.631 kg/株,CI为2.971;腐殖酸施用量和CI之间回归关系极显著(P<0.01),其最高施用量为0.083 kg/株,CI为1.989;钾肥和腐殖酸施用量分别为0.600和0.070 kg/株时,CI达最大值,为4.706。【结论】 钾肥和腐殖酸配施会影响核桃果实的糖代谢酶活性、糖组分含量及品质相关指标。基于MFCE方法建立的综合评价模型表明钾肥和腐殖酸对核桃果实CI具有显著的互作效应,当钾肥和腐殖酸施用量分别为0.600和0.070 kg/株时,CI达最大值,该配施方案可作为新疆地区核桃种植的适宜施肥方案。

     

    Abstract: 【Objective】 This study aimed to employ a multi-level fuzzy comprehensive evaluation (MFCE) method to investigate the effects of potassium fertilizer and humic acid interactions on glycometabolic enzymes and quality of walnut fruits, providing theoretical references for fertilization practices for walnut orchards in Xinjiang. 【Method】 The Wen 185 walnut was used as the experimental material, and the experiment adopted a randomized block design. Four potassium fertilizer (K) levels were set, which were 0 kg/plant (K0), 0.3 kg/plant (K1), 0.6 kg/plant (K2), and 0.9 kg/plant (K3); four humic acid (H) levels were set, 0 kg/plant (H0), 0.05 kg/plant (H1), 0.075 kg/plant (H2), and 0.1 kg/plant (H3); a total of 16 treatments were set. Appearance and internal quality indicators, sugar component content, and activities of glucometabolic enzymes of walnut fruits were determined, and the MFCE method was used to establish a comprehensive evaluation model of walnut fruit quality, with comprehensive index (CI) calculated, to analyze effects of potassium ferti-lizer and humic acid interactions on walnut fruit quality. 【Result】 Range analysis showed that: longitudinal diameter and lateral diameter at K0 level were the largest, single fruit weight at K1 level was the highest; activities of glycometabolic enzymes, sugar components, and soluble sugar content at K2 level were the highest; crude protein content at K3 level was the highest and transverse diameter was the largest; sucrose content was the highest at the H1 level; activities of glycometabolic enzymes and contents of fructose, glucose, starch, and soluble sugar at H2 level were the highest; at H3 level, contents of crude fat and crude protein were the highest, transverse and lateral diameters were the largest, and single fruit weight was the highest. Comprehensive evaluation results showed that the T8 treatment had the highest CI of 5.062, followed by the T5 treatment with a CI of 4.733, while the T16 treatment had the lowest CI of 0.472. Regression analysis results indicated a significant regression relationship between potassium fertilizer application rate and CIP<0.05), and the highest application rate was 0.631 kg/plant, corresponding to a CI of 2.971; an extremely significant regression relationship between humic acid application rate and CIP<0.01), and the highest application rate was 0.083 kg/plant, corres-ponding to a CI of 1.989; when the application rates of potassium fertilizer and humic acid were 0.600 and 0.070 kg/plant respectively, the CI reached its maximum of 4.706. 【Conclusion】 The combined application of potassium ferti-lizer and humic acid can improve activities of glycometabolic enzymes, sugar component content, and indicators related to quality. The comprehensive evaluation model based on the MFCE method shows that potassium fertilizer and humic acid have significant interaction effects on CI of walnut fruits. When the application rates of potassium fertilizer and humic acid are 0.600 and 0.070 kg/plant respectively, the CI reaches its maximum, therefore this fertilization regimen is appropriate for walnut planting in Xinjiang.

     

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