Abstract: 【Objective】 This study aimed to investigate the effects of different exogenous selenium （Se） application rates on Se-enriched Chinese milk vetch production， and the effects of Se-enriched Chinese milk vetch incorporation on soil Se speciation， rice growth， as well as Se uptake and accumulation， thereby providing theoretical basis for establi-shing a technical system of indirect exogenous Se application in Se-enriched rice production. 【Method】 In the pot experiment， Se-containing foliar fertilizer was applied once at the flowering stage of Chinese milk vetch， and four treatments of exogenous Se application rates were set： 0 μg/pot （MV-Se0）， 440 μg/pot （MV-Se1）， 880 μg/pot （MV-Se2）， and 1320 μg/pot （MV-Se3）， with the winter fallow-rice mode as control （CK）. Rice was planted after Chinese milk vetch incorporation at the late flowering stage to analyze growth and Se accumulation of Chinese milk vetch， growth and Se nutrition characteristics of rice， and changes in soil Se speciation in the Chinese milk vetch-rice rotation system. 【Result】 Applying exogenous Se foliar fertilizer at flowering stage of Chinese milk vetch effectively increased Se content and accumulation in plants， and Se content and accumulation increased as exogenous Se application rates increased. However， excessive exogenous Se application reduced Chinese milk vetch biomass. Se-enriched Chinese milk vetch incorporation not only enhanced rice yield but also elevated Se content in parts of rice plant. The Se contents in brown rice under MV-Se1， MV-Se2， and MV-Se3 treatments were significantly higher than that of CK（P<0.05，the same below）， ranging from 1.96 to 4.45 times the value of the Chinese national standard GB/T 22499-2008 Rich Selenium Paddy （0.04 mg/kg）， and the proportion of organic Se content was greater than 86% in all treatments. The Se-enriched Chinese milk vetch incorporation increased the proportions of soil soluble Se and exchangeable Se contents， and the proportion of available Se contents under MV-Se1， MV-Se2， and MV-Se3 treatments increased by 8.33%-26.40% compared to CK. At the maturity stage of rice， the utilization rates of exogenous Se in rice grains and plants under MV-Se1， MV-Se2， and MV-Se3 treatments were 0.22%-0.46% and 1.15%-1.72% respectively， with the primary transfer coefficient of rice significantly higher than that of CK. 【Conclusion】 In the Chinese milk vetch-rice rotation system， incorporation of Se-enriched Chinese milk vetch which is used for producing exogenous Se can increase rice yield and Se contents in parts of rice plant without significantly reducing the proportion of organic Se contents in brown rice， proving that it is a suitable technical mode for Se-enriched rice production.