Abstract: 【Objective】 To screen Trichoderma strains with strong antagonistic ability against the Fusarium oxysporum and good environmental adaptability， which could provide strain resources for the development of efficient and stable biocontrol agents for pepper wilt disease.【Method】 Rhizosphere soil samples were collected from healthy pepper plants in fields where peppers were continuously cropped and wilt disease was severe. Trichoderma strains were isolated and purified using the gradient dilution method combined with Trichoderma-selective medium. The strain with the strongest antagonistic effect was screened by the plate confrontation assay. The inhibition effect of its sterile fermentation broth was determined by the colony diameter method. The antagonistic Trichoderma strain was identified based on morphological observation and molecular biology. Single-factor experiments were conducted to optimize the liquid fermentation conditions to enhance the mycelial biomass and inhibitory activity of the fermentation broth， and the control efficacy was evaluated in potted pepper experiments.【Result】 Five Trichoderma strains were isolated and purified from the soil sample， among which strain ML-01 showed the highest inhibition effect against the pepper wilt pathogen， with 81.47% inhibition rate. The inhibition rate of its sterile fermentation broth against pepper wilt pathogen was 58.20%. Through morphological observation combined with molecular biology identification， strain ML-01 was identified as Trichoderma hamatum. The optimized liquid fermentation conditions of strain ML-01 through single-factor experiments were as follows： glucose 40 g， tyrosine 10 g， pH 5， fermented at 150 r/min and 25 °C for 5 d. Under these conditions， the mycelial biomass and the inhibition rate of the sterile fermentation broth reached 38.84×10^-2 g and 66.59% respectively. In the pot experiment， the control efficacy of the strain ML-01 fermentation broth after optimized fermentation against pepper fusarium wilt reached 80.77%.【Conclusion】 Trichoderma hamatum ML-01 exhibits fine biocontrol effects against pepper wilt. It can be used as a candidate strain resource for developing biocontrol agents for pepper wilt disease.