国外引进籼稻品种在云南稻区的抗稻瘟病评价及抗性基因分子检测

Evaluation of introduced foreign indica rice cultivars for blast resistance in Yunnan and molecular detection of resistance genes

  • 摘要: 【目的】 明确国外引进的籼稻品种在云南稻区的稻瘟病抗性水平以及已知抗稻瘟病基因的组成情况,为利用抗性资源选育抗病新品种及发掘新的抗病基因提供参考。【方法】 以258份国外引进的籼稻品种为材料,通过2023—2024年连续2年的田间自然诱发开展穗瘟抗性鉴定,继而对筛选出的高抗穗瘟籼稻品种苗期人工接种17株稻瘟病菌单孢菌株进行室内叶瘟抗性鉴定,并结合4个位点的8个已知抗性基因Piz-tPi2Pi9PibPi1Pita-2PikpPikh的功能分子标记进行靶标基因分子检测。【结果】 258份国外引进的籼稻品种在田间病圃表现高抗、抗病、中抗、中感、感病和高感的分别有19、2、21、77、51和88份。利用供试17株稻瘟病菌单孢菌株进一步对19份高抗的籼稻品种进行苗期室内人工接种鉴定,结果有11份籼稻品种对全部菌株表现抗性。在258份国外引进籼稻品种中,共有125份籼稻品种检测出1个或多个靶标抗性基因,占48.45%,其中,93份品种检测到1个靶标基因、27份品种检测到2个靶标基因、5份品种检测到3个靶标基因;在检测的8个靶标基因中,携带PibPita-2Pi2Pi1PikpPikhPiz-t基因的籼稻品种分别有85、40、15、8、5、5和4份,分别占供试品种的32.95%、15.50%、5.81%、3.10%、1.94%、1.94%和1.55%;在供籼稻品种中,抗性基因Pib的分布频率最高,Piz-tPikpPikh基因的分布频率较低,未检测到Pi9基因。【结论】 从258份国外引进的籼稻品种中筛选出11份在云南表现广谱抗稻瘟病的水稻品种,11份广谱抗稻瘟病籼稻品种可作为优异抗源应用于云南稻区的抗病育种及抗性基因挖掘。

     

    Abstract: 【Objective】 The aim of the study was to clarify the resistance level of the introduced indica rice cultivars from abroad against rice blast disease in the rice-growing areas of Yunnan, as well as the composition of known resistance genes, which could provide reference for the selection and breeding of new disease-resistant varieties using resistance resources, as well as for the discovery of new disease-resistant genes.【Method】 Panicle blast resistance of 258 introduced foreign indica rice cultivars was evaluated through 2 consecutive years (2023-2024) of field screening under natural blast nursery, subsequently, the highly resistant indica rice cultivars against panicle blast were inoculated with 17 single-spore strains of rice blast pathogens at seedling stage, and indoor leaf blast resistance was identified. Molecular detection of eight known resistance genes located at four loci (Piz-tPi2Pi9PibPi1Pita-2Pikp, and Pikh) was performed using functional molecular markers.【Result】 In the blast nursery, 19, 2, 21, 77, 51, and 88 indica rice cultivars were classified as highly resistant, resistant, moderately resistant, moderately susceptible, susceptible, and highly susceptible respectively among the 258 introduced foreign indica rice cultivars. Nineteen highly-resistant indica rice cultivars were subjected to artificial inoculation at the seedling stage using 17 dominant single-spore strains of rice blast pathogen, and 11 indica rice cultivars of them exhibited resistance to all strains. Among the 258 introduced foreign indica rice cultivars, 125 cultivars carried 1 or multiple target resistance genes, accounting for 48.45% of the tested cultivars. Of these, 93 cultivars carried 1 target gene, 27 cultivars carried 2 target genes, and 5 cultivars carried 3 target genes. Among the 8 target genes analyzed, PibPita-2Pi2Pi1PikpPikh, and Piz-t genes were detected in 85, 40, 15, 8, 5, 5, and 4 indica rice cultivars respectively, accounting for 32.95%, 15.50%, 5.81%, 3.10%, 1.94%, 1.94%, and 1.55% of all tested cultivars. Among all detected genes, the resistence gene Pib showed the highest distribution frequency, whereas Piz-t, Pikp, and Pikh genes were present at relatively low frequencies, and Pi9 gene was not detected in any cultivar.【Conclusion】 From 258 introduced foreign indica rice cultivars, 11 cultivars that show broad-spectrum resistance to rice blast disease in Yunnan are selected. These 11 broad-spectrum resistant indica rice cultivars can be used as excellent sources of resistance for disease resistance breeding and the exploration of resistance genes in the rice-growing areas of Yunnan.

     

/

返回文章
返回