园艺地布覆盖对芍药栽培土壤理化性质及根际土壤细菌群落影响

Effects of black ground fabric mulching on soil physicochemical properties and rhizosphere soil bacterial community in Paeonia lactiflora Pall. cultivation

  • 摘要: 【目的】 探究园艺地布覆盖对芍药园土壤理化性质及根际土壤细菌群落的影响,以期为园艺地布在芍药田间栽培中的应用提供理论依据。【方法】 以芍药品种珊瑚落日为试验材料,设露地栽培(对照)与园艺地布覆盖2个处理,于2022年1—12月采用环刀法采集土壤样品,测定土壤理化性质指标,并利用16S rRNA基因高通量测序分析根际土壤细菌群落结构及其多样性。【结果】 园艺地布覆盖对土壤的温度和湿度均具有一定影响,与对照相比,可显著降低6—8月的土壤温度,降幅为3.77%~4.26%;而在其他月份可提高土壤温度,增幅为4.42%~21.95%;土壤湿度在1—12月均高于对照,增幅为2.97%~535.67%。园艺地布覆盖可降低土壤pH、土壤容重和土壤紧实度,提高土壤电导率(EC)和土壤孔隙度。韦恩图结果显示,园艺地布覆盖下芍药根际土壤细菌OTU数量为4906,较对照提高3.8%。基于门、纲、目分类水平对芍药根际土壤细菌群落结构进行分析发现,园艺地布覆盖可整体提高细菌群落的相对丰度。其中,在门分类水平上,疣微菌门(Verrucomicrobiota)增幅最大,较对照提高118.96%,酸杆菌门(Acidobacteriota)增幅最小,较对照提高0.45%;在纲分类水平上,硝化螺旋菌纲(Nitrospira)增幅最大,较对照提高21.48%,γ-变形菌纲(Gamma proteobacteria)增幅最小,较对照提高3.37%;在目分类水平上,亚硝化单胞菌目(Nitrosomonadales)增幅最大,较对照提高36.06%,粘球菌目(Myxococcales)增幅最小,较对照提高4.19%。Alpha多样性分析结果显示,园艺地布覆盖下的ACE指数、Chao1指数、香农指数和辛普森指数均高于对照。Beta多样性分析和主坐标分析结果显示,园艺地布覆盖下芍药根际土壤细菌群落结构与对照存在明显差异。【结论】 园艺地布覆盖可改善芍药园土壤理化性质,提高根际土壤细菌群落相对丰度,可在芍药大田栽培中推广使用。

     

    Abstract: 【Objective】 This study aimed to investigate the effects of black ground fabric mulching on the soil physicochemical properties and rhizosphere soil bacterial community in Paeonia lactiflora Pall. orchards, thereby providing theoretical basis for the application of black ground fabric in field cultivation of Paeonia lactiflora Pall. 【Method】 The peony cultivar Paeonia lactiflora Shanhuluori was used as the test material. Two treatments were set up: open-field cultivation (control) and black ground fabric mulching. Soil samples were collected using the ring knife method to determine soil physicochemical indicators from January to December in 2022. Furthermore, 16S rRNA gene high-throughput sequencing technology was employed to analyze the structure and diversity of the bacterial community in the rhizosphere soil. 【Result】 Black ground fabric mulching had certain regulatory effect on both soil temperature and moisture. Compared with the control, it significantly reduced soil temperature from June to August, with a decrease ranging from 3.77% to 4.26%; while in the other months, it increased soil temperature by 4.42% to 21.95%. Soil moisture was consistently higher than that of the control throughout the year from January to December, with an increase ranging from 2.97% to 535.67%. Furthermore, black ground fabric mulching decreased soil pH, soil bulk density, and soil compactness, while increasing soil electrical conductivity (EC) and soil porosity. Venn diagram analysis showed that the number of bacterial OTU in the rhizosphere soil under black ground fabric mulching was 4906, which was 3.8% higher than that of control. Analysis of the bacterial community structure at the phylum, class, and order levels revealed that black ground fabric mulching generally increased the relative abundance of the bacterial community. Specifically, at the phylum level, Verrucomicrobiota exhibited the largest increase, increased by 118.96% compared with control, while Acidobacteriota showed the smallest increase, increased by 0.45% compared with control. At the class level, Nitrospira showed the largest increase, increased by 21.48% compared with control, and Gamma proteobacteria showed the smallest increase, increased by 3.37% compared with control. At the order level, Nitrosomonadales showed the largest increase, increased by 36.06% compared with control, and Myxococcales showed the smallest increase, increased by 4.19% compared with control. Alpha diversity analysis indicated that the ACE index, Chao1 index, Shannon index, and Simpson index under black ground fabric mulching were all higher than those of control. Beta diversity analysis and PCoA analysis demonstrated that the rhizosphere soil bacterial community structure under black ground fabric mulching was greatly different from that of control. 【Conclusion】 Black ground fabric mulching can improve the soil physicochemical properties of Paeonia lactiflora Pall. orchards and increase the relative abundance of rhizosphere soil bacterial community. Therefore, it is worthy of promotion and application in the field cultivation of Paeonia lactiflora Pall.

     

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