不同微生物菌剂对羊粪堆肥腐熟特性及温室气体排放的影响

Effects of different microbial agents on decomposition characteristics and greenhouse gas emissions of sheep manure composting

  • 摘要: 【目的】 探究微生物菌剂对羊粪好氧堆肥腐熟过程中理化指标和温室气体排放的影响,为羊粪好氧堆肥的温室气体减排技术提供理论支持。【方法】 试验设4个处理组:CK,不接种微生物菌剂对照组;A组,接种金山菌剂;B组,接种欧克菌剂;C组,接种自配液体微生物菌剂。每个处理组菌剂接种量为10 mL/kg。堆肥周期42 d,每7 d翻堆1次,于第0、7、14、28和42 d采集堆体样品,分析理化性质;同时采用静态箱法每隔1 d采集气体,监测气体排放特征。【结果】 与CK相比,A和C组的堆肥高温期延长8 d。第42 d堆肥结束时,C组的含水率和电导率较CK显著降低(P<0.05,下同),堆肥产品种子发芽指数最高,达127.0%;A和B组的铵态氮含量分别较CK显著或极显著(P<0.01,下同)降低9.35%和14.58%;A和C组的硝态氮含量分别较CK极显著升高43.14%和47.83%;A和C组的总氮含量分别较CK升高5.05%和6.26%;B和C组的总有机碳含量分别较CK升高12.61%和9.59%。与CK相比,第42 d堆肥结束时,A和C组的N2O累计排放量分别降低46.37%和13.20%,CH4累计排放量分别降低51.04%和29.51%;A、B和C组的CO2累计排放量分别提高21.06%、0.87%和38.58%。【结论】 接种金山菌剂和自配菌剂可促进羊粪好氧堆肥腐熟过程中堆体升温,延长堆肥过程的高温期,有效降低电导率,减少氮素损失,并降低N2O和CH4排放,从而减轻温室气体环境污染风险;接种欧克菌剂能有效保留堆体内碳素,但会增加N2O和CH4排放。综合比较,接种自配菌剂在羊粪好氧堆肥腐熟中的效果较佳。

     

    Abstract: 【Objective】 This study aimed to investigate the effects of microbial agents on physicochemical indicators and greenhouse gas emissions during aerobic composting of sheep manure, providing theoretical support for technologies for reducing greenhouse gas emissions from aerobic composting of sheep manure. 【Method】 Four treatment groups were set up in the experiment: CK, a control group without microbial agent inoculation; Group A, inoculated with Jinshan microbial agent; Group B, inoculated with Ouke microbial agent; Group C, inoculated with a self-developed liquid microbial agent. The inoculation amount for each treatment group was 10 mL/kg. The composting period lasted for 42 d, with turning for every 7 d. Samples were collected on the 0th, 7th, 14th, 28th, and 42nd d, to analyze their physicochemical properties. Meanwhile, gases were collected at intervals of 1 d using the static chamber method for monitoring gas emission characteristics. 【Result】 Compared with CK, Group A and Group C extended the high-temperature period by 8 d. At the end of composting on the 42nd d, the water content and conductivity of Group C were significantly lower than those of CK (P<0.05, the same below), and the seed germination index of compost product was the highest, reaching 127.0%. The ammoniacal nitrogen contents in Group A and Group B were significantly or extremely significantly (P<0.01, the same below) reduced by 9.35% and 14.58%, respectively, compared to CK. The nitrate nitrogen contents in Group A and Group C were significantly increased by 43.14% and 47.83% respectively. Total nitrogen contents in Group A and Group C increased by 5.05% and 6.26% respectively,compared to CK, while total organic carbon content in Group B and Group C increased by 12.61% and 9.59% respectively,compared to CK. Compared with CK, at the end of composting on the 42nd d, the cumulative N2O emissions in Group A and Group C decreased by 46.37% and 13.20%, respectively, and cumulative CH4 emissions decreased by 51.04% and 29.51%, respectively. The cumulative CO2 emissions in Group A,Group B, and Group C increased by 21.06%, 0.87%, and 38.58% respectively. 【Conclusion】 Inoculation with the Jinshan microbial agent and the self-developed microbial agent during aerobic composting of sheep manure can promote temperature rise in the compost, extend the high-temperature period, effectively reduce electrical conductivity, minimize nitrogen loss, and decrease N2O and CH4 emissions, thereby mitigating the environmental pollution risk of greenhouse gases. Inoculation with the Ouke microbial agent can effectively retain carbon in the compost but increase N2O and CH4 emissions. Overall, the self-developed microbial agent demonstrates superior performance in the aerobic composting of sheep manure.

     

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