Abstract:
【Objective】 This study aimed to investigate the effects of
Panax notoginseng （Burk.） F. H. Chen-
Bletilla striata-
Panax notoginseng （Burk.） F. H. Chen rotation cropping under forest on alleviating continuous cropping obstacles of
Panax notoginseng （Burk.） F. H. Chen and potential cross-infection risks between pathogens of the two plants， and to evaluate the effects of
Panax notoginseng （Burk.） F. H. Chen root-zone soil on growth， quality， and disease of
Bletilla striata for elucidating feasibility of
Panax notoginseng （Burk.） F. H. Chen-
Bletilla striata-
Panax notoginseng （Burk.） F. H. Chen
rotation cropping， thereby providing a theoretical basis for screening suitable rotation crops that could alleviate continuous cropping obstacles of
Panax notoginseng （Burk.） F. H. Chen to achieve sustainable
Panax notoginseng （Burk.） F. H. Chen cultivation. 【Method】
Bletilla striata was cultivated in continuous
Panax notoginseng （Burk.） F. H. Chen cropping soil and natural soil under forest， and the differen-ces in growth parameters， quality， and disease of
Bletilla striata planted in the two kinds of soil were measured to investigate the effects of continuous
Panax notoginseng （Burk.） F. H. Chen cropping soil on
Bletilla striata. Continuous
Panax notoginseng （Burk.） F. H. Chen
cropping soil was further utilized for rotation
Bletilla striata cropping， and after that，
Panax notoginseng （Burk.） F. H. Chen was replanted in that soil to determine seedling survival rate， dry weight， and saponin content of
Panax notoginseng （Burk.） F. H. Chen
to evaluate the effects of rotation
Bletilla striata cropping on alleviating subsequent continuous cropping obstacles of
Panax notoginseng （Burk.） F. H. Chen.
Bletilla striata root and leaves were inoculated with pathogens of
Panax notoginseng （Burk.） F. H. Chen root rot and leaves， and
Panax notoginseng （Burk.） F. H. Chen root and leaves were inoculated with pathogens of
Bletilla striata root rot and leaves respectively， to investigate the cross-infection between pathogens of
Panax notoginseng （Burk.） F. H. Chen and
Bletilla striata. Real-time fluorescence quantitative PCR was performed to analyze their common cross-infected pathogens. 【Result】 Compared with natural soil， continuous
Panax notoginseng （Burk.） F. H. Chen cropping soil showed no significant negative effects on growth （plant height， biomass， and chlorophyll content）， quality （total phenol， polysaccharide， and Militarine content）， or disease （anthracnose， rust， and root rot） of
Bletilla striata （
P>0.05）. Replanting
Panax notoginseng （Burk.） F. H. Chen after
Panax notoginseng （Burk.） F. H. Chen-
Bletilla striata rotation
cropping could significantly increase root dry weight of
Bletilla striata （
P<0.05， the same below）， extremely significantly improve seedling survival rate of
Bletilla striata （
P<0.01， the same below） compared with plantation in continuous
Panax notoginseng （Burk.） F. H. Chen cropping soil， and extremely significantly improve contents of total ginsenosides， Rg1， Rb1， R1， and Rd. Both
Panax notoginseng （Burk.） F. H. Chen and
Bletilla striata were susceptible to infection by root rot pathogens of
Ilyonectria spp.； however， the contents of pathogens of
Ilyonectria spp. were significantly decreased after rotation
Bletilla striata cropping followed by continuous
Panax notoginseng （Burk.） F. H. Chen cropping. 【Conclusion】 Continuous
Panax notoginseng （Burk.） F. H. Chen cropping soil under forest exhibits no significant effects on growth， quality， and disease of
Bletilla striata；
Bletilla striata rotation cropping improves
Panax notoginseng （Burk.） F. H. Chen seedling survival rate and quality， as well as reduces contents of pathogens of
Ilyonectria spp. in soil.
Panax notoginseng （Burk.） F. H. Chen-
Bletilla striata-
Panax notoginseng （Burk.） F. H. Chen rotation cropping is feasible under forest. Root rot pathogens cause cross-infection between
Panax notoginseng （Burk.） F. H. Chen and
Bletilla striata， therefore continuous monitoring of potential hazards in large-scale cultivation is recommended.
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