Abstract: 【Objective】 This study aimed to isolate and identify a wild Campanophyllum strain collected from Yunnan， conduct biological characteristic experiments as well as its domestication and cultivation， thereby providing technical references for developing and utilizing germplasm resources of this genus.【Method】 The taxonomic status of the strain was determined through morphological observation and molecular biological identification. A test of biological characteristics was performed based on four single factors： carbon source， nitrogen source， temperature， and pH； three optimal levels of factors were selected from each factor to perform an orthogonal experiment， followed by domestication and cultivation.【Result】 The wild strain grew in clusters or groups on decaying wood. The fruiting bodies were fleshy and semicircular or fan-shaped， with a light clay-yellow pileus surface. The flesh was white or light yellow. The gills were adnate， extremely crowded， and their color turned from white to pale yellow. The stipes were short and lateral， with a light brown to brown coloration. Mycelia from the purified strain appeared light gray with brown pigmentation； the aerial mycelia were dense with radiant margins. These morphological characteristics were consistent with previous reports of Campanophyllum microsporum. Molecular biological analysis showed that the strains ITS and nrLSU sequences were highly similar with Campanophyllum microsporum （HKAS133169） in the NCBI data base； as they clustered into one branch of the phylogenetic tree， it was confirmed to be Campanophyllum microsporum. The biological characteristic experiment showed that， for Campanophyllum microsporum， the optimal carbon source was sucrose， the optimal nitrogen source was yeast extract powder， the optimal temperature was 20 ℃， and the optimal pH was 8.0. The best medium combination was determined by the orthogonal experiment： sucrose as carbon source， ammonium chloride as nitrogen source， temperature of 20 ℃， and pH of 6.7. Fruiting was achieved using a cultivation formula of 78% wood chips， 20% wheat bran， 1% limestone， and 1% gypsum， with a water content of around 60%. At 25 ℃， after 45-55 d primordia were formed and transferred to a mushroom house at 20 ℃ and 85% humidity for further culture of 30-35 d， the fruiting bodies fully matured.【Conclusion】 The wild Campanophyllum strain collected from Yunnan is identified as Campanophyllum microsporum， which is domesticated and cultivated， filling the blank of biological characteristics of domestication and cultivation of this species， and providing germplasm materials for its sustainable development and utilization as well as genetic improvement.