Abstract: 【Objective】 This study aimed to examine biological characteristics and cultivation evaluation of wild Ganoderma lingzhi， providing a reference for developing and utilizing wild Ganoderma lingzhi germplasm resources， as well as breeding and cultivating new varieties.【Method】 The Guangdong wild Ganoderma strain YS-4 was isolated and purified. The strains were identified through morphological observation and ITS sequence analysis. The biological characteristics of strain YS-4 under conditions of different carbon sources， nitrogen sources， temperatures， pH， and inorganic salt were analyzed via single-factor experiments. A four-factor and three-level orthogonal test was employed to screen optimal mycelial growth conditions. With the cultivated strains LZ-8 and LZ-11 as control， the tolerances of strains YS-4， LZ-8， and LZ-11 under heat stress treatments were evaluated to the strains’ comprehensive traits of fruiting bodies and contents of effective components.【Result】 Morphological observation and molecular biological identification confirmed that the tested wild Ganoderma strain YS-4 was Ganoderma lingzhi. The single-factor experiment showed that the optimal carbon source for YS-4 mycelial growth was fructose， the optimal nitrogen source was ammonium nitrate， the optimal inorganic salt was calcium chloride， the optimal pH was 5.0， and the optimal temperature was 30 ℃. Orthogonal test results indicated optimal mycelial growth of strain YS-4， with fructose as carbon source， ammonium nitrate as nitrogen source， calcium carbonate as inorganic salt， and temperature of 25 ℃. Heat stress tests revealed that at 40 ℃， the mycelial growth inhibitory rate reached 100.00% for strains YS-4， LZ-8， and LZ-11， without any mycelial growth. The strain YS-4 exhi-bited the highest mycelial recovery growth rate at 2.70 mm/d， significantly exceeding that of the control strains LZ-8 and LZ-11 （P<0.05）. The cultivation experiment revealed that strain YS-4 exhibited shorter germination period， primordium formation time， and fruiting body maturation of 38， 9， and 42 d respectively， all faster than the strains LZ-8 and LZ-11. The fruiting bodies of strain YS-4 showed a darker color， with its pileus thicknesses of 16.35 mm， pileus length of 1.83 cm， pileus length of 11.25 cm， pileus width of 5.83 cm， fresh weight of 45.01 g， dry weight of 28.87 g， biological efficiency of 3.21%， polysaccharide content of 2.39%， and triterpenoid and sterol content of 1.24%.【Conclusion】 The strain YS-4 exhibits strong heat stress tolerance， a short growth cycle， thick pileus， and high contents of polysaccharide， triterpenoid， and sterol in fruiting bodies， showing it is an excellent wild Ganoderma lingzhi strain suitable for breeding and cultivating new varieties.