不同品种桃果实香气及氨基酸成分分析

Analysis of aroma and amino acid components in fruits of different peach varieties

  • 摘要:目的】探究不同品种桃果实的香气及氨基酸成分,明确桃果实香气成分与风味形成的关系,为新疆桃的品种改良和选育提供参考依据。【方法】以新疆北疆地区9个品种桃果实为研究对象,分别利用热脱附—气相色谱—质谱联用(ATD-GC-MS)和高效液相—色谱—质谱联用(HPLC-MS/MS)技术检测桃果实的香气成分和氨基酸含量,并对香气成分相对含量与氨基酸含量进行相关分析。【结果】9个品种桃果实中共检测出61种香气成分,其中脂类物质在香气成分中占比最高,主要呈果香;乙酸乙酯、苯乙酮、苯甲醛、十六烷、十四烷和十五烷为9个品种桃果实共有的香气成分;白蟠17号香气成分种类最多,共25种,伊犁白桃24种,水蜜桃23种,英勒尔蟠桃21种,白油桃和小黄油各20种,黄油蟠和黄蟠23号各19种,黄油桃香气成分种类最少,共17种。从9个品种桃果实中共检测出16种氨基酸,不同种类氨基酸含量差距较大,氨基酸总含量最高的为黄油蟠,最低的为黄油桃;天冬氨酸在9个品种桃果实中的含量均最高,其次为丙氨酸、丝氨酸和谷氨酸;水蜜桃的支链氨基酸、芳香族氨基酸和增香与着色氨基酸含量均较高。在扁平形的桃果实中,酯类物质相对含量与丙氨酸含量呈显著(P<0.05,下同)正相关;醇类物质相对含量与赖氨酸含量呈极显著(P<0.01,下同)负相关,与谷氨酸和苯丙氨酸含量呈显著负相关;醛类物质相对含量与谷氨酸、苯丙氨酸和赖氨酸含量呈显著负相关。在圆形的桃果实中,醇类物质相对含量与丝氨酸含量呈极显著正相关,与蛋氨酸含量呈显著正相关;酮类物质相对含量与苯丙氨酸含量呈显著正相关。【结论】从9个品种桃果实中共鉴定出61种香气成分,其中酯类为主要香气物质,主要呈果香,对桃果实风味起决定作用;共检测出16种氨基酸,以天冬氨酸含量最高;氨基酸与香气成分相关,对桃果实风味形成具有潜在影响。

     

    Abstract:Objective】This study aimed to investigate the aroma and amino acid components, elucidate the relationship between aroma components and flavor formation of peach fruits, providing references for improving and breeding peach varieties from Xinjiang.【Method】Fruits of nine peach varieties from the northern region of Xinjiang were taken as research objects. The automatic thermal desorption-gas chromatography-mass spectrometry (ATD-GC-MS) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) were used to determine aroma components and amino acid content, and correlation analysis was performed on relative contents of aroma components and amino acid content.【Result】A total of 61 aroma components were detected in the fruits of nine peach varieties. Among them, esters showed the highest proportion among aroma components, mainly presenting fruity notes. Ethyl acetate, acetophenone, benzaldehyde, hexadecane, tetradecane, and pentadecane were the common aroma components of fruits of nine peach varieties. The most abundant aroma was found in Baipan No. 17, with a total of 25 types; Ili White Peach had 24 aroma types, Honey Peach had 23 types, Yingler Flat Peach had 21 types, White Nectarine had 20 types, Xiao Huangyou had 20 types, Huangyou Nectarine had 19 types, Yellow Nectarine No. 23 had 19 types; Huangyou Peach had the least aroma components of 17. A total of 16 amino acids were found in fruits of nine peach varieties, with different types of amino acids showing a large difference. The highest content of total amino acids was found in Huangyou Nectarine, and the lowest was found in Huangyou Peach. Aspartic acid showed the highest contents in the fruits of nine peach varieties, followed by alanine, serine, and glutamic acid. Honey Peach had higher contents of branched-chain amino acids, aromatic amino acids, and amino acids related to aroma enhancement and coloring. In flat peach fruits, esters had significant positive correlations with alanine content (P<0.05, the same below); relative contents of alcohols had significant extremely negative correlation with lysine content (P<0.01, the same below), and significant negative correlations with contents of glutamic acid and phenylalanine. Relative contents of aldehydes had significant negative correlations with glutamic acid, phenylalanine, and lysine. In round peach fruits, the relative content of alcohols showed a highly significant positive correlation with serine content, and a significant positive correlation with methionine content; the relative content of ketones exhibited a significant positive correlation with phenylalanine content.【Conclusion】From the fruits of nine peach varieties, 61 aroma components are identified, and esters were the main aroma substances decisive of peach fruit flavor, presenting fruity notes; 16 amino acids are detected, and aspartic acid had the highest content; amino acids are correlated with aroma components, showing a potential influence on peach fruit flavor formation.

     

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