Abstract:
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Objective】This study aimed to identify and comprehensively evaluate saline-alkali tolerance of 95 broad bean germplasms at germination stage, and screen broad bean germplasms with strong saline-alkali tolerance, so as to provide references for identification of saline-alkali tolerant broad bean germplasm and breeding of saline-alkali tolerant varieties.【
Method】8.0 g/L compound saline (NaCl, Na
2CO
3, and Na
2SO
4 with mass ratio of 9∶1∶1, pH of 9.25) was used to pose saline-alkali stress to 95 broad bean germplasms to determine seven indexes at germination stage: relative germination index, relative germination potential, relative germination rate, relative radicle length, relative radicle weight, relative germ length, and relative germ weight. Correlation analysis, principal component analysis, membership function analysis, cluster analysis, and stepwise regression were used to comprehensively evaluate the saline-alkali tole-rance of 95 broad bean germplasms.【
Result】Under compound saline-alkali stress, coefficients of variation of relative germination index, relative germination potential, relative germination rate, relative radicle length, relative radicle weight, relative germ length, and relative germ weight were more than 10.00%; the coefficients of variation of relative radicle length and relative radicle weight exceeded 200.00%, indicating that these two indexes were highly unstable under saline-alkali stress. Most of the saline-alkali tolerance indexes in broad bean germplasms exhibited positive correlations: ten pairs of indexes showed extremely significant positive correlations (
P<0.01), and one pair showed a significant positive correlation (
P<0.05); the strongest correlation was observed between relative germ length and relative germ weight, with a correlation coefficient of 0.968, while the weakest correlation was between relative germination rate and relative germ length, with a correlation coefficient of -0.026. The seven saline-alkali tolerance indexes were reduced to three principal components, with a cumulative contribution rate of 89.588%. Based on the comprehensive evaluation
D values, the 95 broad bean germplasms were classified into five categories: saline-alkali intolerant (3 germplasms, 3.16%), low saline-alkali intolerant (14 germplasms, 14.74%), moderately saline-alkali intolerant (45 germplasms, 47.37%),saline-alkali tolerant (24 germplasms, 25.26%), and highly saline-alkali tolerant (9 germplasms, 9.47%). Relative germination index, relative germination potential, relative germination rate, and relative radicle length could be used as primary indexes for evaluating saline-alkali tolerance in broad bean. Among the top 10 germplasms ranked by the comprehensive evaluation
D value and the top 10 ranked by the total membership function value, eight were identical. Similarly, among the bottom 10 germplasms ranked by the comprehensive evaluation
D value and the bottom 10 ranked by the total membership function value, seven were identical, indicating that the two methods yielded similar results in evaluating the saline-alkali tolerance of broad bean germplasms.【
Conclusion】Indexes related to saline-alkali tolerance of 95 broad bean germplasms show abundant variation. Nine highly saline-alkali tolerant broad bean germplasm resources are screened, which can be used as parental materials for breeding saline-alkali tolerant broad bean. Relative germination index, relative germination potential, relative germination rate, and relative radicle length can be used as primary indexes for identifying saline-alkali tolerance of broad bean.