Abstract: 【Objective】 This study aimed to screen and identify plant growth-promoting rhizobacteria （PGPR） from Lycium barbarum rhizosphere，and analyze the effects of their growth and plant growth-promoting characteristics on Lycium barbarum seeds，thereby providing a scientific basis of PGPR development and application for Lycium barbarum.【Method】 Rhizosphere soil samples of four Lycium barbarum varieties were used as test materials to isolate and purify bacterial strains via the plate streaking method. Molecular identification of the strains was performed using 16S rDNA sequence analysis. The growth characteristics of the strains were examined under different temperatures， NaCl concentrations， pH levels， and sodium selenite concentrations. The phosphate-solubilizing ability，indole-3-acetic acid （IAA） production ability，and nitrogen-fixing ability of the strains were determined. The antagonistic effects among the strains were assessed. The effects of PGPR on germination rate and growth-promotion for Lycium barbarum were investigated using single and dual bacterial treatments.【Result】 Seventeen rhizobacterial strains were isolated and purified from the four Lycium barbarum varieties， belonging to five genera：Bacillus （12 strains），Pseudomonas （2 strains）， Priestia （1 strain）， Niallia （1 strain）， and Mammaliicoccus （1 strain）. According to the rhizobacterial growth characteristics under different conditions，the suitable growth conditions for the 17 strains were： temperature 28-55 ℃ ， NaCl concentration 0%-5.0%，pH 6-8， and sodium selenite concentration 5%-15%. Detection results of phosphate-solubilizing ability， IAA production ability，and nitrogen-fixing ability showed that：twelve strains had phosphate-solubilizing ability；strain D38 showed the highest IAA production， reaching a content of 5.06 mg/L； and six strains （D7， D17， D19， D32， D34， and D38） had nitrogen-fixing ability. Antagonism experiments revealed that antagonistic interactions were found between strains D38 and D34，and between D32 and D34. Seed germination experiments indicated that，compared with CK，the germination rates of Lycium barbarum seeds treated with D38 alone and with D38+D32 combination reached 63.33% and 93.33%，respectively. Pot experiments demonstrated that the PGPR increased the indexes including plant height， root length， stem length， fresh weight，and dry weight of Lycium barbarum； compared with CK， dual bacterial treatments increased plant height by 15.267-45.067 mm， stem length by 8.733-15.867 mm，fresh weight by 0.089-0.133 g，and dry weight by 0.007-0.012 g.【Conclusion】 Twelve phosphate-solubilizing strains and 6 nitrogen-fixing strains are isolated from the rhizosphere soil of Lycium barbarum. Strains D17， D32 ，D34， and D38 significantly promote seed germination and plant growth，and the dual bacterial treatment D38+D32 shows the best effect，which could provide bacterial resources and theoretical references for the microbial fertilizer development of Lycium barbarum.