复合肥和菌剂对草豆蔻生长、光合作用和养分积累的影响

Effects of compound fertilizer and microbial inoculum on growth, photosynthesis, and nutrient accumulation of Alpinia hainanensis seedlings

  • 摘要: 【目的】 探究不同施肥处理对草豆蔻(Alpinia hainanensis)幼苗生长发育、光合特性及养分吸收利用的影响,为指导草豆蔻苗期科学施肥提供参考。【方法】 以3个月生草豆蔻实生苗(初始苗高8.9 cm、株丛数1个)为试验材料,采用盆栽试验,设置2.5 g/株复合肥(C1)、5.0 g/株复合肥(C2)、5.0 g/株菌剂(S)、2.5 g/株菌剂+2.5 g/株复合肥(M1)、1.0 g/株菌剂+4.0 g/株复合肥(M2)共5个复合肥和菌剂处理,以不施肥处理为对照(CK),测定草豆蔻幼苗的生长指标、光合指标及养分含量,并对各指标进行相关分析和模糊隶属函数分析。【结果】 不同复合肥和菌剂处理下草豆蔻幼苗的多数指标表现优于CK,且大多与CK差异显著(P<0.05)或极显著(P<0.01);单施复合肥处理(C2)效果优于单施菌剂处理(S),合理的菌剂、复合肥配施效果优于二者单施。复合肥单施(C1、C2)和配施(M1、M2)处理中,随着复合肥施肥量增加,草豆蔻幼苗地上部分生长指标、干物质积累、光合指标及养分含量均有不同程度提升。相关分析结果表明,草豆蔻幼苗施用复合肥及菌剂后的生长指标、干物质积累与养分含量间密切相关,其中根、叶的养分含量与其他指标间相关性较强,而茎的全碳(TC)、全氮(TN)、全钾(TK)含量与其他指标间的相关性大多低于根和叶。各处理的隶属度均值排序依次为M2>C2>M1>C1>S>CK。【结论】 合理的菌剂与复合肥配施处理能有效促进草豆蔻幼苗生长与干物质积累,提升其光合能力,并促进营养元素积累,以1.0 g/株菌剂+4.0 g/株复合肥处理的效果最佳。建议在草豆蔻苗期培育过程中重视氮素的供应及利用,并配施适宜的益生菌剂,以更好地促进草豆蔻幼苗的生长发育。

     

    Abstract: 【Objective】 This study aimed to explore the effects of different fertilization treatments on the growth, photosynthesis characteristics and nutrient uptake and utilization of Alpinia hainanensis seedlings,so as to provide a reference for fertilization of the plant at seedling stage.【Method】 Three-month-old Alpinia hainanensis seedlings (initial plant height of 8.9 cm and plant number of 1) were used as test materials in the a pot experiment. Five compound fertilizer and microbial inoculum treatments were set: 2.5 g/plant compound fertilizer(C1),5.0 g/plant compound fertilizer(C2),5.0 g/plant microbial inoculum(S),2.5 g/plant microbial inoculum+2.5 g/plant compound fertilizer(M1),and 1.0 g/plant microbial inoculum+4.0 g/plant compound fertilizer(M2) treatments. Non-fertilization treatment was taken as the control(CK). The growth indexes,photosynthesis indexes, and nutrient contents were determined to conduct correlation analysis and fuzzy membership function analysis on each index.【Result】 Under the treatment of different compound fertilizers and microbial inocula,most indexes of Alpinia hainanensis seedlings were better than those of CK,and most treatment groups showed significant differences (P<0.05)or extremely significant differences(P<0.01). Effects of applying compound fertilizer alone (C2) were better than that of applying microbial agent inoculum (S),and effects of appropriate combined application of microbial inoculum and compound fertilizer were better than that of applying any of them alone. In the treatment of single application (C1 and C2) and combined application (M1 and M2) of fertilizers, with the increase of compound fertilizer application dosage,the above-ground part growth indexes,dry matter accumulation,photosynthesis indexes, and nutrient contents were all improved to varying degrees. Correlation analysis results showed that: the growth indexes, dry matter accumulation, and nutrient content of Alpinia hainanensis seedlings under compound fertilizer and microbial inoculum treatments were closely correlated; the nutrient content in root and leaf had a strong correlation with other indexes, while the total carbon (TC), total nitrogen (TN), and total potassium (TK) contents in stem had a relatively strong correlation with other indexes, which was lower than that of root and leaf. The ranking of the average membership degrees of each treatment from largest to smallest was M2>C2>M1>C1>S>CK.【Conclusion】 Appropriate combined application of microbial inoculum and compound fertilizer effectively promotes the growth and dry matter accumulation of Alpinia hainanensis seedlings, enhances their photosynthetic ability, and facilitate nutrient accumulation. The treatment of 1.0 g/plant of microbial inoculum+4.0 g/plant of compound fertilizer shows the best effects. During the cultivation of Alpinia hainanensis seedlings,attention should be paid to its nitrogen supply and utilization,and proper probiotic inocula should be applied to promote the growth and development of Alpinia hainanensis seedlings.

     

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