赣南茶区10个引种茶树品种的品质成分及多样性分析
Analysis of quality components and diversity of ten cultivars of introduced tea plants in the Gannan tea area
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摘要: 【目的】 分析江西赣南茶区引种10个茶树品种的品质成分及多样性,为赣南茶区引种适制狗牯脑茶品种及新品种选育提供参考。【方法】 以赣南茶区引种的10个茶树品种鲜叶为材料,以福鼎大白茶为对照进行物候期观测,并测定10个品种茶样品质成分,采用主成分分析和聚类分析对其品质成分和遗传多样性进行评价,并分析其适制性。【结果】 物候期观测结果显示,黄金茶1号属于特早生品种,中黄3号和中茶108属于早生品种,其余7个茶树品种与对照品种的物候期相差较小。10个引种茶树品种的品质成分含量存在一定差异,其中水分含量3.98~8.81 g/100 g,水浸出物含量46.67%~53.83%,茶多酚含量19.70%~26.93%,游离氨基酸总量2.50%~3.80%,咖啡碱含量1.90%~3.70%,儿茶素总量7.95%~18.40%,表儿茶素(EC)含量0.48%~1.12%,表儿茶素没食子酸酯(ECG)含量1.27%~3.46%,表没食子儿茶素(EGC)含量0.47%~2.78%,表没食子儿茶素没食子酸酯(EGCG)含量4.90%~10.54%,儿茶素(C)含量0.16%~2.11%,酯型儿茶素含量6.17%~14.00%,非酯型儿茶素含量1.42%~4.40%,儿茶素品质指数400.00~1526.15。浙农117的儿茶素总量及水浸出物、茶多酚、ECG、EGCG、C、酯型儿茶素和非酯型儿茶素含量等8个指标均最高。10个引种茶树品种均属于高水浸出物资源,且浙农117和中茶108属于高儿茶素资源,御金香属于低咖啡碱资源。10个引种茶树品种遗传多样性指数为2.12~2.30,平均2.26;变异系数为0.00%~57.19%,平均22.96%,其中C的变异系数最高(57.19%)。对10个引种茶树品种进行聚类分析,当遗传距离为7.5时可分为两大类群,第Ⅰ类群包括6个茶树品种,表现为咖啡碱含量、游离氨基酸总量和儿茶素总量较高,儿茶素品质指数较低;第Ⅱ类群包括4个茶树品种,表现为水浸出物含量、茶多酚含量和儿茶素品质指数较高,EC含量较低。主成分分析15个指标,提取前4个成分为主成分,累计方差贡献率达95.68%。10个引种茶树品种中,奶白茶、御金香、中黄1号、中黄2号和黄金芽5个品种适制绿茶,浙农113、黄金茶1号、中茶108和中黄3号4个品种表现为红茶和绿茶兼制,浙农117适制红茶。【结论】 江西赣南茶区引种的10个茶树品种化学成分丰富,具有较高的水浸出物含量,并存在高儿茶素、低咖啡碱等特异性资源,同时遗传多样性指数与变异系数均较高。10个引种茶树品种在特色茶产品开发与遗传改良方面具有进一步研究和开发利用的价值,其中浙农117有适制狗牯脑茶的潜力。Abstract: 【Objective】 This study aimed to analyze quality components and diversity of ten cultivars of introduced tea plants in Gannan tea area of Jiangxi Province, and to provide references for introducing suitable tea cultivars for produ-cing Gougunao tea and breeding new tea cultivars in the Gannan tea area.【Method】 Fresh leaves of ten cultivars of tea plants introduced to the Gannan tea area were taken as materials, and Fuding Dabaicha was taken as the control to observe their phenological phases. Quality components of samples in the ten cultivars were also determined. Principal component analysis and cluster analysis were used to evaluate their quality components, genetic diversity, and suitability.【Result】 Phenological observation indicated that Huangjincha No. 1 was classified as an extra-early sprouting cultivar, whereas Zhonghuang No. 3 and Zhongcha 108 were early-sprouting cultivars; while the phenological phases of the remaining seven tea cultivars showed minor differences compared to the control. The ten cultivars of introduced tea plants showed differences in contents of quality components: water of 3.98-8.81 g/100 g, water extract of 46.67%-53.83%, tea polyphenol of 19.70%-26.93%, total free amino acid of 2.50%-3.80%, caffeine of 1.90%-3.70%, and total catechin of 7.95%- 18.40%, epicatechin (EC) of 0.48%-1.12%, epicatechin gallate (ECG) of 1.27%-3.46%, epigallocatechin (EGC) of 0.47%-2.78%, epigallocatechin gallate (EGCG) of 4.90%-10.54%, catechin (C) of 0.16%-2.11%, ester catechin 6.17%- 14.00%, non-ester catechin 1.42%-4.40%, with the catechin quality index of 400.00-1526.15. Zhenong 117 showed eight highest indexes, including total catechin, water extract, tea polyphenol, ECG, EGCG, C, ester catechin, and non-ester catechin. The ten cultivars of introduced tea plants were resources of high water extracts, Zhenong 117 and Zhongcha 108 were resources of high catechin, and Yujinxiang were resources of low caffeine. For the ten cultivars of introduced tea plants, genetic diversity indexes ranged from 2.12-2.30 (mean of 2.26), coefficients of variation 0.00%-57.19% (mean of 22.96%), with the highest coefficient of variation found in C (57.19%). Cluster analysis of the ten cultivars of introduced tea plants showed that: at the genetic distance of 7.5, they could be divided into two groups; the GroupⅠincluded six tea cultivars with higher contents of caffeine, total free amino acid, and total catechin and a lower catechin quality index; the Group Ⅱ included four tea cultivars with higher contents of water extract, tea polyphenol and catechin quality index and a lower EC content. Among the 15 indexes of principal component analysis, the first four were extracted as principal components, with their cumulative variance contribution rate of 95.68%. Among the ten cultivars of introduced tea plants, five varieties (Naibai tea, Yujinxiang, Zhonghuang No. 1, Zhonghuang No. 2, and Huangjingya) demonstrated suitability for green tea production; four cultivars (Zhenong 113, Huangjincha No. 1, Zhongcha 108, and Zhonghuang No. 3) exhibited suitability for both black and green tea production; while Zhenong 117 exhibited suitability for black tea production.【Conclusion】 The ten tea cultivars introduced to Gannan tea area enjoy rich chemical components, demonstrating high water extract content, and specific resources with high catechin and low caffeine are found among them, showing relatively high genetic diversity indexes and coefficients of variation. The ten cultivars of introduced tea plants exhibit considerable potential for further research and utilization in the development of specialty tea products and genetic improvement. Zhenong 117 shows potential suitability for producing Gougunao tea.
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