河西走廊地区梨砧穗组合嫁接亲和性综合评价

Comprehensive evaluation of grafting compatibility of rootstock-scion combinations of pear in the region of Hexi Corridor

  • 摘要: 【目的】 研究不同梨矮化砧穗组合嫁接亲和性并进行综合评价,为筛选适宜河西走廊地区栽培梨矮化砧穗组合,加快当地矮化中间砧木的应用推广提供参考依据。【方法】 以杜梨为基砧,中矮1号为矮化中间砧木,以早酥梨、早酥红梨、新梨7号、红南果梨、京白梨、长把梨和香水梨为接穗分别设置7个砧穗组合(T1~T7组合),进行嫁接试验。测定不同砧穗组合梨嫁接苗成活率、砧穗生长量等生长指标及叶片的光合参数。通过主成分分析和聚类分析及隶属函数法对各砧穗组合的嫁接亲和性进行综合评价。【结果】 不同砧穗组合梨嫁接成活率均大于90.00%,排序为T1组合>T3组合>T6组合>T2组合>T4组合>T7组合>T5组合,其中T1组合成活率为97.34%;T5组合的成活率为94.78%。不同砧穗组合梨中间砧/接穗周长比为1.03~1.30,新梢长度为76.37~133.87 cm,愈合状况最好的组合为T1,其中间砧与接穗周长比为1.03;T7组合叶片最多,新梢最长,中间砧直径和接穗直径净生长量高于其他组合。不同砧穗组合中,梨嫁接苗T7组合的胞间CO2浓度的日均值最低,仅为189.972 μmol/mol;净光合速率的日均值最高,达5.460 µmol/(m2·s),显著高于其他砧穗组合(P<0.05,下同);气孔限制值的日均值最高,为0.531;水分利用率的日均值最小,仅为1.739 µmol/mol。7个砧穗组合梨嫁接苗叶片净光合速率的日变化整体上均呈“M”形变化趋势。相关分析结果显示,接穗直径净生长量与叶片数、中间砧直径净生长量与基砧直径净生长量、基砧直径净生长量与净光合速率均呈显著正相关,相关系数分别为0.79、0.84、0.85。3个主成分累计方差贡献率达85.391%。隶属函数法分析结果显示,各砧穗组合嫁接亲和性排序为T7组合>T6组合>T5组合>T3组合>T1组合>T2组合>T4组合。聚类分析将7个砧穗组合分为两类,T1和T7组合聚为一类。【结论】 筛选出适合河西走廊地区矮化密植栽培的梨嫁接组合是香水梨作为接穗,中矮1号作为矮化中间砧木。

     

    Abstract: 【Objective】 This study aimed to investigate and comprehensively evaluate the graft compatibility of diffe-rent dwarf rootstock-scion combinations of pear, in order to screen suitable combinations for the Hexi Corridor region and provide a reference for promoting the application and extension of dwarf interstocks in local pear cultivation. 【Method】 Using Pyrus betulifolia as the rootstock, and Zhongai 1 as the dwarf interstock, grafting experiments were conducted with seven rootstock-scion combinations of pear cultivars: Zaosu, Zaosu Red, Xinli 7, Hongnanguo, Jingbai, Changba, and Xiangshui, forming seven rootstock-scion combinations (T1-T7). Indexes such as survival rate, rootstock-scion growth, and leaf photosynthetic parameters of the grafted pear seedlings of different rootstock-scion combinations were measured. Principal component analysis (PCA), cluster analysis, and membership function method were employed for a comprehensive evaluation of graft compatibility. 【Result】 The graft survival rates of all combinations exceeded 90.00%, ranked as T1>T3>T6>T2>T4>T7>T5. The T1 combination had the highest survival rate at 97.34%, while T5 had the lo-west at 94.78%. The circumference ratio of interstock/scion across different combinations ranged from 1.03 to 1.30, and new shoot length ranged from 76.37 to 133.87 cm. The T1 combination showed the best healing status with an interstock-scion circumference ratio of 1.03. The T7 combination had the most leaves, the longest new shoots, and the net growth of interstock and scion diameter was higher than in other combinations. Among the different combinations, the T7 grafted seedlings had the lowest daily average intercellular CO2 concentration (189.972 μmol/mol); the highest daily average net photosynthetic rate 5.460 nmol/(m2·s), which was significantly higher than other combinations (P<0.05, the same below); T7 also had the highest daily average stomatal limitation value (0.531) and the lowest daily average water use efficiency 1.739 μmol/mol. The daily variation of leaf net photosynthetic rate in all seven combinations generally exhibited a bimodal “M” curve. Correlation analysis revealed positive correlations between the net growth of scion diameter and leaf number, between net growth of interstock diameter and net growth of base stock diameter, and between net growth of base stock diameter and net photosynthetic rate, with their correlation coefficients of 0.79, 0.84, and 0.85. The cumulative variance contribution rate of the three principal components reached 85.391%. Membership function analysis showed that the graft compatibility of the combinations ranked as: T7>T6>T5>T3>T1>T2>T4. Cluster analysis grouped the seven combinations into two categories, with T1 and T7 as one category. 【Conclusion】 The graft combination suitable for high-density dwarf cultivation in the Hexi Corridor region was identified as using the pear cultivar Xiangshui as the scion, and Zhongai 1 as the dwarf interstock.

     

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