Abstract: 【Objective】 This study aimed to identify the key pathogens causing root rot in Angelica sinensis from Weiyuan， Gansu， and to study its effects on the rhizosphere soil microorganisms and metabolites of Angelica sinensis， so as to provide a theoretical basis for research and development of biocontrol agents for root rot of Angelica sinensis. 【Method】 Angelica sinensis from Weiyuan， Gansu was used as the research subjects. The pathogens causing root rot were isolated and purified using tissue separation method， and they were identified using morphological and molecular biological methods. The lethal concentrations of the pathogens were determined through pot experiments， and the disease model was established using the optimal solution concentration. The changes in the rhizosphere soil microorganisms and metabolites of Angelica sinensis under the stress of pathogen were analyzed using 16S rDNA， ITS rDNA high-throughput sequencing technology， and liquid chromatography–mass spectrometry （LC-MS） methods. 【Result】 Ten strains were isolated and purified from root tissues of diseased Angelica sinensis. Among which， the strain Z was identified as the pathogen. Based on the morphological characteristics and molecular biological features of strain Z， it was identified as Fusarium solani. Under the effect of strain Z， the rhizosphere soil microbial community structure of Angelica sinensis changed significantly at the genus level： 29 bacterial genera showed extremely significant inter-group differences between the healthy group and diseased group （P<0.01，the same below）， and they were non-dominant genera in the two groups except for Gemmatimonadaceae_unclassified； 7 fungal genera showed extremely significant inter-group differences， and all of them were dominant genera in the diseased group. There were 14 significantly different rhizosphere soil metabolites between the diseased group and the healthy group （P<0.05， the same below）， and the contents of taurine， quinolinic acid， 6-phosphogluconic acid， and oxoadipic acid were significantly up-regulated in the diseased group. Through Pearson correlation analysis of differential microorganisms at P<0.01 and differential metabolites in rhizosphere soil of Angenlica sinensis showed that the significantly up-regulated taurine， quinolinic acid， 6-phosphogluconic acid， and oxoadipic acid in the diseased group had significant or extremely significant positive correlations with 10 bacterial genera， including Blfdi19， KD3-10， Luteolibacter， Saccharimonas， Aquicella， Arenimonas， Ferruginibacter， Opitutus， A4b， and Dyadobacter， but there were no significant correlations among the differential fungi （P>0.05）. 【Conclusion】 Fusarium solani is the key pathogen causing root rot in Angelica sinensis from Weiyuan， Gansu. Interference of Fusarium solani leads to obvious changes in rhizosphere soil microbial community structure and metabolites of Angelica sinensis. The impact of the pathogen is stronger on the fungal genus structure than on the bacterial genus structure， but the differential metabolites have significant or extremely significant correlations with the differential bacterial genera. Taurine， quinolinic acid， 6-phosphogluconic acid， and oxoadipic acid may be potential “cry for help” substances； Luteolibacter， Saccharimonas， Arenimonas， Ferruginibacter， Opitutus， A4b， and Dyadobacter may be potential beneficial bacteria recruited.