Abstract: Objective This study aimed to investigate effects of different bamboo sawdust substrates on growth qua-lity and antioxidant activities of Sparassis latifolia， with the goal of expanding the sources of main materials for Sparassis latifolia cultivation and realizing the high-value utilization of scraps produced during bamboo product processing.Method In the single-factor test， Sparassis latifolia was used as subjects bamboo sawdust was used to partially replace the main material of pine sawdust in the conventional formula for Sparassis latifolia factory production. Treatments of bamboo sawdust gradient additions （12%， 24%， 36%， 48%， 60%） were set up and marked as A， B， C， D， and E treatments. The conventional production substrate formula （78% pine sawdust） was used as the control （CK）. The growth indicators such as mycelial growth， primordia formation， fruiting body yield， and the quality indicators such as contents of protein， total amino acids， crude fiber， crude polysaccharides， and ergosterol in Sparassis latifolia fruiting bodies were evaluated using the membership function method. The correlation between the growth and quality indicators of Sparassis latifolia was studied through correlation analysis， and effects of crude polysaccharide production on radical scavenging rates.Result The tested Sparassis latifolia strains could grow normally under different bamboo sawdust substrate treatments， with mycelial growth rates ranging from 1.4 to 2.1 mm/d. Treatments A， B， and C， as well as the control （CK）， exhibited good uniformity in primordium differentiation， with primordia showing a clean white color， dense distribution， and vigorous growth， reaching an adherent growth height of 3-5 cm. The fruiting body yield ranged from 94.3 to 211.3 g/bag. The comprehensive evaluation of the growth and quality indicators of Sparassis latifolia showed that the optimal formula was treatment C； under the treatment C， the mycelial growth rate of the tested strain was 2.1 mm/d， the primordia formation duration was 33 d， and the fruiting body yield was 211.3 g/bag， which were all better than CK and had good commercial characteristics. For the growth and quality indicators in the comprehensive evaluation of Sparassis latifolia strains under bamboo sawdust substrate treatments， the order was treatment C > treatment B > treatment D > treatment A > CK > treatment E. The crude polysaccharides of the fruiting bodies in treatment C could better scavenge 1,1-diphenyl-2-picrylhydrazyl （DPPH）， 2,2'-azino-bis（3-etilbenzotiazolin）-6-sulfonic acid （ABTS） and O₂^- than those in treatment B and CK. Under the concentration of 4.0 mg/mL， the scavenging rates of treatment C reached 81.4%， 57.3%， and 60.1%， respectively.Conclusion Partially replacing pine sawdust in conventional production could increase fruiting body yield， the content of nutrients， and effects of crude polysccharides production radical-scavenging rate. The suitable substrate for Sparassis latifolia cultivation is 36% bamboo sawdust， 42% pine sawdust， 10% wheat flour， 10% corn flour， 1.5% brown sugar， and 0.5% peptone.