Abstract: Objective This study aimed to characterize the mitochondrial genome structure of Aeolothrips fasciatus， and investigate its phylogenetic relationships based on its mitochondrial genome sequences， so as to provide molecular evidence for clarification of the phylogenetic relationships and taxonomic status of various groups within Aeolothripidae.Method The mitochondrial genome of Aeolothrips fasciatus was sequenced using next-generation sequencing （NGS） of the Illumina NovaSeq 6000 platform. The genome sequences were annotated and analyzed. A dataset matrix was established based on the 13 protein-coding genes （PCGs） and 22 transfer RNA （tRNA） genes from the mitochondrial genome of 50 species of Thysanoptera. With Hyalopterus arundiniformis as the outgroup， the phylogenetic tree was established employing both maximum likelihood （ML） and Bayesian inference （BI） methods to analyze the polygenic relationship between Aeolothrips fasciatus and other thrip species.Result The complete mitochondrial genome of Aeolothrips fasciatus was 19167 bp in length， comprising of 37 genes—13 PCGs， 22 tRNA genes， 2 ribosomal RNA （rRNA） genes—along with 2 non-coding control regions （CRs）. Within the coding regions of Aeolothrips fasciatus， 10 gene overlaps and 21 intergenic spacers were identified. The genome of Aeolothrips fasciatus exhibited typical mitochondrial genome compositions and arrangements of Thysanoptera species， with its AT content of 71.5%， indicating a pronounced AT skew. In the 13 PCGs of mitochondria in Aeolothrips fasciatus， gene cox3 took GTG as the start codon， gene nad3 took TTG as the start codon， the rest 11 PCGs took ATN as the start codon. For the stop condons， genes atp6 and nad1 had their stop codons ended with T， the gene nad2 took TAG as its stop codon， the rest 10 PCGs took TAA as their stop condons. The gene trnH lacked the TφC ring， and trnS1 lacked the DHC arm， but the other 20 tRNA genes exhibited a complete clover-shape structure. Extensive gene rearrangements were observed， with at least 26 genes showed translocation. Phylogenetic analysis showed that Aeolothrips fasciatus was cluster with Aeolothrips indicus in the same branch.Conclusion The complete mitochondrial genome sequence of Aeolothrips fasciatus is obtained. The mitochondrial genome structure of Aeolothrips fasciatus is consistent with mitochondrial genomes of Aeolothripidae insects. Phylogenetic analysis shows that Aeolothrips fasciatus shares a near relationship with Aeolothrips indicus. Aeolothripidae itself could form a stable monophyly.