分子标记辅助选择聚合Pi2Xa23基因改良广恢1002抗病性

Improvement of Guanghui 1002 resistance by polymerizing Pi2 and Xa23 genes through molecular marker-assisted selection

  • 摘要: 【目的】通过聚合Pi2Xa23抗性基因改良广恢1002的稻瘟病和白叶枯病抗性,为保持优异恢复系及深入挖掘强优势杂交稻组合潜力提供技术参考。【方法】以华南稻区应用面积最大的迟熟弱感光恢复系广恢1002为改良对象,结合传统育种手段与分子标记辅助选择,将抗稻瘟病基因Pi2和抗白叶枯病基因Xa23一次性导入广恢1002,对筛选出的5个抗性改良系进行抗性鉴定、农艺性状测定和稻米品质考察,并将其与吉丰A进行组配,分析其杂种优势。【结果】根据分离群体的分子标记检测结果,综合田间农艺性状的表现,从BC4F4代群体筛选出5个纯合抗性改良系(R1002-1、R1002-2、R1002-3、R1002-4和R1002-5)。经考察发现,5个抗性改良系与广恢1002遗传相似性达97.62%以上,在主要农艺性状(穗长、有效穗数、穗粒数、结实率、千粒重和产量)方面与广恢1002无显著差异(P>0.05,下同),但稻瘟病抗性频率从61.5%增至79.5%~94.9%。白叶枯病抗性大幅增强,对Ⅲ、Ⅳ、Ⅴ和Ⅸ型白叶枯菌均表现为高抗,其中R1002-1和R1002-3米质较好,达部优二等。5个抗性改良系与吉丰A组配杂种F1代与吉丰优1002在主要农艺性状(株高、穗长、有效穗数、穗粒数、结实率、产量)方面无显著差异,米质等级均为普通。【结论】5个广恢1002抗稻瘟病和白叶枯病改良系的遗传背景回复达到预期,在不减产的前提下各改良系稻瘟病和白叶枯病抗性大幅增强,且部分改良系稻米品质有所提升,实现了改良目的。后续可代替现有广恢1002组配出稻瘟病和白叶枯病抗性更好的杂交水稻品种。

     

    Abstract: 【Objective】The resistance to rice blast and bacterial blight of Guanghui 1002 was improved by polymerizing the Pi2 and Xa23 resistance genes, providing technical reference for maintaining elite restorer lines and further exploring the potential of strong dominant hybrid rice combinations. 【Method】Using Guanghui 1002—the most widely cultivated late-maturing,weak-photosensitive restorer line in southern China—as the recipient parent,simultaneously introduced the blast resistance gene Pi2 and bacterial blight resistance gene Xa23 via molecular marker-assisted selection(MAS) combined with conventional breeding. Five improved resistant lines were selected for disease resistance evaluation,agronomic trait assessment and grain quality analysis. These lines were then crossed with Jifeng A to analyze their heterosis performance. 【Result】Based on molecular marker detection and field agronomic performance of isolated populations,5 homozygous resistant improved lines(R1002-1, R1002-2, R1002-3, R1002-4 and R1002-5) were selected from the BC4F4 population. The 5 homozygous resistant improved lines exhibited over 97.62% genetic similarity with Guanghui 1002,with no significant difference(P>0.05) in major agronomic traits(spike length,effective spike number, grain number per spike, seed setting rate, 1000-grain weight and yield). However,their blast resistance frequency improved from 61.5% to 79.5%-94.9%,and bacterial blight resistance was greatly enhanced,showing high resistance to types Ⅲ,Ⅳ,Ⅴ and Ⅸ of Xanthomonas oryzae pv. oryzae. Notably,R1002-1 and R1002-3 demonstrated improved grain quality,reaching the “Ministry Standard Grade 2” level. When the 5 homozygous resistant improved lines crossed with Jifeng A,the F1 hybrids showed no significant differences in agronomic traits(plant height,spike length,effective spike number, grain number per spike, seed setting rate and yield), and the rice quality was ordinary. 【Conclusion】The genetic background recovery of 5 Guanghui 1002 improved lines resistant to rice blast and bacterial blight has met expectations. Without reducing production, the resistance of each improved line to rice blast and bacterial blight has greatly increased, and the rice quality of some improved lines has improved, achieving the improvement goal. Subsequently, these lines can replace the existing Guanghui 1002 to develop hybrid rice varieties with better resistance to rice blast and bacterial blight.

     

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