Abstract: 【Objective】 To explore the effects of light，nitrogen fertilizer and their interaction on the leaf structure of Erythropalum scanden cuttings，which could provide scientific basis for the study of the ratio of light and nitrogen suita-ble for the growth and development of Erythropalum scanden leaves and the cultivation of strong seedlings of Erythropalum scanden. 【Method】 Taking the cuttings of 2-year-old Erythropalum scanden as the research object，4 light transmittance levels［100.00% natural light（NS）（L0），70.85% NS（L1），52.45% NS（L2），13.85% NS（L3）］and 5 nitrogen levels（0，2.5，5.0，7.5 and 10.0 g/plant urea）were set，marked as N0，N1，N2，N3 and N4 respectively，with 100.00% NS and 0 g/plant urea as the control（L0N0），there were a total of 20 combination treatments of light and nitrogen. Leaf anatomical structures of Erythropalum scandens under different treatments were observed to analyze and compare the effects on leaf thickness，cuticle thickness，epidermal thickness，and mesophyll structure. A comprehensive analysis of the impacts of light-nitrogen interactions on leaf anatomy was conducted. 【Result】 The leaf tissue structure of Erythropalum scandens was arranged sequentially as follows：upper cuticle，upper epidermis，palisade tissue，spongy tissue，lower epidermis and lower cuticle. The main effect of light had extremely significant impact on leaf thickness of Erythropalum scandens（P<0.01，the same below）while the main effect of nitrogen significantly affected leaf thickness（P<0.05，the same below）. The highest leaf thickness of Erythropalum scandens（114.20 μm）was observed in L0N0，which was extremely significantly higher than all nitrogen treatments under L2 and L3. Under the same nitrogen level，leaf thickness and upper cuticle thickness generally decreased with reduced light intensity. Moreover，the L0 full light treatment was greater than other light treatments，the thickness of the spongy tissue initially decreased and then increased. Under the same light intensity，with the increase of nitrogen level，lower epidermal thickness first increased and then decreased with increasing nitrogen levels in L0 and L1 light environments. Palisade tissue thickness under L0 and L1 light environments also followed the changing trend of increasing first and then decreasing，while no significant changes were observed under L2 and L3 light environments（P>0.05）. The palisade tissue/spongy tissue ratio mirrored the trend of palisade tissue thickness. Light-nitrogen interactions significantly affected palisade tissue thickness of Erythropalum scandens. Light had extremely significant main effect on palisade tissue/spongy tissue ratio，while both nitrogen main effect and light-nitrogen interactions showed extremely significant effects on palisade tissue/spongy tissue ratio. The results of the fuzzy membership function showed that the average ranking of the membership functions of the 20 light and nitrogen treatment groups as follows： L0N3 > L1N2 > L0N4 > L0N1 > L1N4 > L0N2 > L1N1 > L1N3 > L1N0 > L2N4 > L3N4 > L0N0 > L2N3 > L2N2 > L3N3 > L3N2 > L2N0 > L2N1 > L3N1 > L3N0. 【Conclusion】 The combination of high-intensity light and moderate nitrogen fertilization（100.00% NS + 7.5 g/plant urea）is optimal for leaf growth of Erythropalum scandens cutting seedlings.