Abstract:
【Objective】 To study the species and biological characteristics of the pathogen causing ginger(
Zingiber officinale)white scab disease,and to screen effective fungicides for inhibiting mycelium growth of the pathogen in the laboratory,which could provide basis for field control of ginger white scab disease. 【Method】 Diseased ginger leaves were col‐lected from a ginger plantation in Laifeng County,Enshi Prefecture,Hubei Province. The pathogen was isolated and purified by tissue separation method,and pathogenicity was assessed based on Koch’s rule. Sequences of ITS,
EF-1α,
ACT,
CAL,
TUB2,and
GAPDH of the pathogen were amplified using specific primers. The pathogen was identified by combining morphological characteristics and molecular identification. The biological characteristics of the pathogen and the inhibitory effects of 8 commonly used fungicides on mycelium growth were evaluated using the mycelium growth rate method. 【Result】 Five strains of pathogens with consistent morphology were isolated and purified from the diseased ginger leaves. The representative strain 23-5 was selected for morphological and molecular biological identification. Its morphological characteristics were consistent with those of
Neoscirrhia matteucciicola;after comparative analysis,the reference strain sequences with high coverage and similarity were selected. The phylogenetic tree was constructed after splicing the ITS-
TUB2 sequence. The results showed that strain 23-5 and
Neoscirrhia matteucciicola were clustered in the same branch. The pathogen of ginger white scab disease was determined to be
Neoscirrhia matteucciicola. The determination results of the biological characteristics of the pathogen indicated that the lethal temperature of the pathogen was 54 ℃;the mycelium growth was more suitable at pH of 6 to 8. When the pH was 7,the colony diameter was the largest,reaching 6.45 cm. The optimal carbon source for pathogen was soluble starch,and the suitable nitrogen sources were peptone, yeast extract and beef extract powder. Among the 8 tested fungicides,difenoconazole had the best inhibitory effect on the mycelium growth of strain 23-5,with an inhibitory medium concentration(EC
50)of 0.05 mg/L. 【Conclusion】 This study is the first to identify
Neoscirrhia matteucciicola as the pathogen of ginger white scab disease in Hubei Province. Difenoconazole demonstrates a good inhibitory effect on the mycelium growth of this pathogen and can be recommended as a candidate fungicide for field control of ginger white scab disease.