Abstract: 【Objective】 To screen the genes related to detoxification metabolism of Agriolimax agrestis under tea saponin stress，and explore the resistance mechanism of Agriolimax agrostis to biological pesticides，which could provide scien-tific basis for the study of drug resistance mechanism and drug resistance management of Agriolimax agrostis. 【Method】 In this study，Illumina high-throughput sequencing technology was used to sequence the transcriptome（RNA-Seq） of Agriolimax agrestis in tea saponin and blank control treatments. The functional annotation，functional classification and metabolic pathway analysis of differentially expressed genes（DEGs）were performed using bioinformatics softwares such as Trinity，DESeq2 and GOseq，and the screened drug-resistant related genes were verified by real-time fluorescence quantitative PCR. 【Result】 The results showed that 1359 DEGs were screened from the transcriptome data，including 586 significantly up-regulated genes（P<0.05，the same below），773 significantly down-regulated genes. A total of 51 DEGs related to insect drug resistance were screened，involving metabolic resistance genes such as glutathione S-transferase （GST），cytochrome P450 （CYP450） and carboxylesterase （CarE）. GO functional annotation analysis showed that DEGs were mainly related to oxidoreductase activity，transcription of DNA templates，organelle pathways and DNA binding. A total of 232 DEGs were mapped to 129 different metabolic pathways in KEGG，mainly involving in antigen processing and presentation，protein processing fluid shear stress in endoplasmic reticulum and atherosclerosis， drug metabolism-cytochrome P450 and other metabolic pathways closely related to insect resistance. The results of real-time fluorescence quantitative PCR of drug resistance related genes were consistent with those of transcriptome，which indicated that the sequencing data of transcriptome was accurate and reliable. 【Conclusion】 The Agriolimax agrestis tea saponin stress are analyzed by transcriptome sequencing technology，and detoxification metabolism-related genes such as GST，cytochrome P450 and CarE are mined. These genes play a key role in the stress response of Agriolimax agrestis when facing the threat of tea saponin and are the core factors of their drug resistance.