Abstract: 【Objective】 To analyze the genetic diversity of phenotypic traits of wild sugarcane germplasm resources in Yongzhou，Hunan，which could provide new genetic resources for sugarcane genetic breeding. 【Method】 A total of 99 wild sugarcane germplasm resources of 5 species in 4 genera were collected from 51 villages（towns）of 11 counties （cities，districts）in Yongzhou. According to the industry standard，26 phenotypic traits（6 quantitative traits and 20 quali‐tative traits）were measured，and the coefficient of variation，Shannon-Wiener diversity index（H'）and correlation coefficient between quantitative traits were calculated. Finally，cluster analysis was carried out based on quantitative traits. 【Result】 A total of 99 germplasm resources were collected from the investigation of wild sugarcane germplasm resources in Yongzhou，Hunan，including 37 Narenga porphyrocoma，28 Saccharum spontaneum，20 Erianthus arundinaceus，12 Miscanthus sinensis and 2 Miscanthus floridulus. The wild sugarcane germplasm resources in Yongzhou were mainly distributed in the altitude of 83.0-549.0 m，24°34′00″N-27°35′38″N，111°09′03″E-112°35′58″E. The interspecific variation of wild sugarcane germplasm resources was abundant，and the average coefficient of variation was 25.0%-35.1%. The average coefficient of variation of N. porphyrocoma was the largest，which was 35.1%，and the average coefficient of variation of S. spontaneum was the smallest，which was 25.0%. There was no significant correlation between internode length and leaf length，stalk length and brix（P>0.05，the same below），and there was no significant correlation between brix and leaf length，plant height. The H ′of quality traits of 99 sugarcane wild germplasm resources ranged from 0 to 1.3086. Cluster analysis and excellent resource screening showed that 2023HBW212 of N. porphyrocoma Ⅳ group， 2023GSM206 and 2023GSM007 of S. spontaneum Ⅰ group，2023BM204 of E. arundinaceus Ⅲ group and 2023M204 of M. sinensis Ⅰ group had good yield and quality traits. 【Conclusion】 The collection of wild sugarcane germplasm resources fills the gap in the systematic investigation of Guangxi Sugarcane Germplasm Repository（Nanning ）in Yongzhou，Hunan. The 99 wild sugarcane germplasm resources collected have rich genetic diversity of phenotypic traits，and 5 germplasms with excellent comprehensive performance are screened out，which have breeding and utilization potential.